EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.61 | PdcG with His6 tag inserted into expression vectors pET30a and expressed in Escherichia coli BL21 (DE3) cells | Pseudomonas sp. |
1.3.1.32 | expressed in Escherichia coli | Pseudomonas sp. |
1.13.11.66 | expressed in Escherichia coli as His-tagged fusion proteins | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.61 | EDTA | 5 mM, 83.2% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | SDS | 5 mM, 81.6% activity compared to untreated control | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.61 | Ba2+ | 5 mM chloride salt, 123.3% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Cd2+ | 5 mM sulfate salt, 58.9% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Co2+ | 5 mM chloride salt, 147.1% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Cu2+ | 5 mM chloride salt, 110.2% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Fe2+ | 5 mM chloride salt, 39.5% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Fe3+ | 5 mM chloride salt, 187.4% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | K+ | 5 mM chloride salt, 95.7% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Mg2+ | 5 mM chloride salt, 138.1% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Mn2+ | 5 mM chloride salt, 186.1% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Na+ | 5 mM chloride salt, 88.2% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Ni2+ | 5 mM chloride salt, 183.9% activity compared to untreated control | Pseudomonas sp. | |
1.2.1.61 | Zn2+ | 5 mM chloride salt, 145.9% activity compared to untreated control | Pseudomonas sp. | |
1.3.1.32 | Ba2+ | 129.02% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Cd2+ | 39.52% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Co2+ | 79.14% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Cu2+ | 59.23% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | EDTA | 112.93% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Fe2+ | 118.90% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Fe3+ | 134.89% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | K+ | 129% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Mg2+ | 129.79% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Mn2+ | 136.59% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Na+ | 123.91% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Ni2+ | 35.25% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | SDS | 136.59% activity compared to no addition 100% | Pseudomonas sp. | |
1.3.1.32 | Zn2+ | 21.44% activity compared to no addition 100% | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | 52000 | - |
recombinant His6-PdcG by SDS-PAGE | Pseudomonas sp. |
1.13.11.66 | 18000 | - |
SDS-PAGE | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.37 | benzene-1,2,4-triol + O2 | Pseudomonas sp. | - |
maleylacetate | - |
? | |
1.13.11.37 | benzene-1,2,4-triol + O2 | Pseudomonas sp. 1-7 | - |
maleylacetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.61 | Pseudomonas sp. | - |
isolated from methyl parathion polluted activated sludge | - |
1.2.1.61 | Pseudomonas sp. 1-7 | - |
isolated from methyl parathion polluted activated sludge | - |
1.3.1.32 | Pseudomonas sp. | C1KF11 | - |
- |
1.3.1.32 | Pseudomonas sp. 1-7 | C1KF11 | - |
- |
1.13.11.37 | Pseudomonas sp. | - |
isolated from methyl parathion-polluted activated sludge, gene pdcC encoded in gene cluster pdcEDGFCBA | - |
1.13.11.37 | Pseudomonas sp. 1-7 | - |
isolated from methyl parathion-polluted activated sludge, gene pdcC encoded in gene cluster pdcEDGFCBA | - |
1.13.11.66 | Pseudomonas sp. | - |
- |
- |
1.14.13.166 | Pseudomonas sp. | D2STN9 | - |
- |
1.14.13.166 | Pseudomonas sp. 1-7 | D2STN9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.61 | His6-PdcG by Ni2+-NTA affinity chromatography | Pseudomonas sp. |
1.13.11.66 | using Ni-NTA chromatography | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | 0.44 | - |
purified recombinant His6-PdcG enzyme, pH 8.0 at 50°C | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.61 | 4-hydroxymuconic semialdehyde + NAD+ + H2O | - |
Pseudomonas sp. | maleylacetate + NADH + 2 H+ | - |
? | |
1.2.1.61 | 4-hydroxymuconic semialdehyde + NAD+ + H2O | - |
Pseudomonas sp. 1-7 | maleylacetate + NADH + 2 H+ | - |
? | |
1.3.1.32 | 2-maleylacetate + NADH | - |
Pseudomonas sp. | 3-oxoadipate + NAD+ | - |
? | |
1.3.1.32 | 2-maleylacetate + NADH | - |
Pseudomonas sp. 1-7 | 3-oxoadipate + NAD+ | - |
? | |
1.13.11.37 | benzene-1,2,4-triol + O2 | - |
Pseudomonas sp. | maleylacetate | - |
? | |
1.13.11.37 | benzene-1,2,4-triol + O2 | - |
Pseudomonas sp. 1-7 | maleylacetate | - |
? | |
1.13.11.66 | hydroquinone + O2 | - |
Pseudomonas sp. | 4-hydroxymuconic acid semialdehyde | - |
? | |
1.13.11.66 | hydroquinone + O2 | - |
Pseudomonas sp. 1-7 | 4-hydroxymuconic acid semialdehyde | - |
? | |
1.14.13.166 | 4-nitrocatechol + NAD(P)H + H+ + O2 | - |
Pseudomonas sp. | 2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O | - |
? | |
1.14.13.166 | 4-nitrocatechol + NAD(P)H + H+ + O2 | - |
Pseudomonas sp. 1-7 | 2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.61 | 4-HS dehydrogenase | - |
Pseudomonas sp. |
1.2.1.61 | 4-hydroxymuconic semialdehyde dehydrogenase | - |
Pseudomonas sp. |
1.2.1.61 | PdcG | - |
Pseudomonas sp. |
1.3.1.32 | maleylacetate reductase | - |
Pseudomonas sp. |
1.3.1.32 | pdcF | - |
Pseudomonas sp. |
1.13.11.37 | PdcC | - |
Pseudomonas sp. |
1.13.11.66 | hydroquinone 1,2-dioxygenase | - |
Pseudomonas sp. |
1.13.11.66 | PdcDE | - |
Pseudomonas sp. |
1.14.13.166 | p-nitrophenol monooxygenase-II | - |
Pseudomonas sp. |
1.14.13.166 | PdcA | - |
Pseudomonas sp. |
1.14.13.166 | PNP 4-monooxygenase | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | 50 | - |
- |
Pseudomonas sp. |
1.3.1.32 | 40 | - |
- |
Pseudomonas sp. |
1.13.11.66 | 40 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | 20 | 70 | - |
Pseudomonas sp. |
1.3.1.32 | 20 | 70 | - |
Pseudomonas sp. |
1.13.11.66 | 20 | 70 | - |
Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | additional information | - |
purified enzyme retains 65% activity after 20 min at 60°C | Pseudomonas sp. |
1.3.1.32 | 60 | - |
enzyme retains 10% activity after 20 min at 60°C | Pseudomonas sp. |
1.13.11.66 | 60 | - |
the purified enzyme retains 35% activity after 20 min at 60°C | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | 8 | - |
- |
Pseudomonas sp. |
1.3.1.32 | 7 | - |
- |
Pseudomonas sp. |
1.13.11.66 | 6 | - |
- |
Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | 5 | 10 | - |
Pseudomonas sp. |
1.3.1.32 | 5 | 9 | - |
Pseudomonas sp. |
1.13.11.66 | 3 | 10 | - |
Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | 3 | 10 | 18% activity after 30 min at pH 3.0, and 75% activity after 30 min at pH 10.0 | Pseudomonas sp. |
1.3.1.32 | 10 | - |
enzyme retains 58% activity after 30 min at pH 10 | Pseudomonas sp. |
1.13.11.66 | 3 | - |
20% activity after 30 min at pH 3.0 | Pseudomonas sp. |
1.13.11.66 | 10 | - |
60% activity after 30 min at pH 10.0 | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.61 | NAD+ | - |
Pseudomonas sp. | |
1.3.1.32 | NADH | - |
Pseudomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.1.61 | metabolism | enzyme is part of the degradation pathway of 4-nitrophenol which is a priority environmental pollutant | Pseudomonas sp. |
1.13.11.37 | metabolism | the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol inthe route via 4-nitrocatechol, overview | Pseudomonas sp. |