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Literature summary extracted from

  • Zhang, S.; Sun, W.; Xu, L.; Zheng, X.; Chu, X.; Tian, J.; Wu, N.; Fan, Y.
    Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in Pseudomonas sp. 1-7 (2012), BMC Microbiol., 12, 27.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.61 PdcG with His6 tag inserted into expression vectors pET30a and expressed in Escherichia coli BL21 (DE3) cells Pseudomonas sp.
1.3.1.32 expressed in Escherichia coli Pseudomonas sp.
1.13.11.66 expressed in Escherichia coli as His-tagged fusion proteins Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.61 EDTA 5 mM, 83.2% activity compared to untreated control Pseudomonas sp.
1.2.1.61 SDS 5 mM, 81.6% activity compared to untreated control Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.61 Ba2+ 5 mM chloride salt, 123.3% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Cd2+ 5 mM sulfate salt, 58.9% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Co2+ 5 mM chloride salt, 147.1% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Cu2+ 5 mM chloride salt, 110.2% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Fe2+ 5 mM chloride salt, 39.5% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Fe3+ 5 mM chloride salt, 187.4% activity compared to untreated control Pseudomonas sp.
1.2.1.61 K+ 5 mM chloride salt, 95.7% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Mg2+ 5 mM chloride salt, 138.1% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Mn2+ 5 mM chloride salt, 186.1% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Na+ 5 mM chloride salt, 88.2% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Ni2+ 5 mM chloride salt, 183.9% activity compared to untreated control Pseudomonas sp.
1.2.1.61 Zn2+ 5 mM chloride salt, 145.9% activity compared to untreated control Pseudomonas sp.
1.3.1.32 Ba2+ 129.02% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Cd2+ 39.52% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Co2+ 79.14% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Cu2+ 59.23% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 EDTA 112.93% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Fe2+ 118.90% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Fe3+ 134.89% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 K+ 129% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Mg2+ 129.79% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Mn2+ 136.59% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Na+ 123.91% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Ni2+ 35.25% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 SDS 136.59% activity compared to no addition 100% Pseudomonas sp.
1.3.1.32 Zn2+ 21.44% activity compared to no addition 100% Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.61 52000
-
recombinant His6-PdcG by SDS-PAGE Pseudomonas sp.
1.13.11.66 18000
-
SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.37 benzene-1,2,4-triol + O2 Pseudomonas sp.
-
maleylacetate
-
?
1.13.11.37 benzene-1,2,4-triol + O2 Pseudomonas sp. 1-7
-
maleylacetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.61 Pseudomonas sp.
-
isolated from methyl parathion polluted activated sludge
-
1.2.1.61 Pseudomonas sp. 1-7
-
isolated from methyl parathion polluted activated sludge
-
1.3.1.32 Pseudomonas sp. C1KF11
-
-
1.3.1.32 Pseudomonas sp. 1-7 C1KF11
-
-
1.13.11.37 Pseudomonas sp.
-
isolated from methyl parathion-polluted activated sludge, gene pdcC encoded in gene cluster pdcEDGFCBA
-
1.13.11.37 Pseudomonas sp. 1-7
-
isolated from methyl parathion-polluted activated sludge, gene pdcC encoded in gene cluster pdcEDGFCBA
-
1.13.11.66 Pseudomonas sp.
-
-
-
1.14.13.166 Pseudomonas sp. D2STN9
-
-
1.14.13.166 Pseudomonas sp. 1-7 D2STN9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.61 His6-PdcG by Ni2+-NTA affinity chromatography Pseudomonas sp.
1.13.11.66 using Ni-NTA chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.61 0.44
-
purified recombinant His6-PdcG enzyme, pH 8.0 at 50°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.61 4-hydroxymuconic semialdehyde + NAD+ + H2O
-
Pseudomonas sp. maleylacetate + NADH + 2 H+
-
?
1.2.1.61 4-hydroxymuconic semialdehyde + NAD+ + H2O
-
Pseudomonas sp. 1-7 maleylacetate + NADH + 2 H+
-
?
1.3.1.32 2-maleylacetate + NADH
-
Pseudomonas sp. 3-oxoadipate + NAD+
-
?
1.3.1.32 2-maleylacetate + NADH
-
Pseudomonas sp. 1-7 3-oxoadipate + NAD+
-
?
1.13.11.37 benzene-1,2,4-triol + O2
-
Pseudomonas sp. maleylacetate
-
?
1.13.11.37 benzene-1,2,4-triol + O2
-
Pseudomonas sp. 1-7 maleylacetate
-
?
1.13.11.66 hydroquinone + O2
-
Pseudomonas sp. 4-hydroxymuconic acid semialdehyde
-
?
1.13.11.66 hydroquinone + O2
-
Pseudomonas sp. 1-7 4-hydroxymuconic acid semialdehyde
-
?
1.14.13.166 4-nitrocatechol + NAD(P)H + H+ + O2
-
Pseudomonas sp. 2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O
-
?
1.14.13.166 4-nitrocatechol + NAD(P)H + H+ + O2
-
Pseudomonas sp. 1-7 2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.61 4-HS dehydrogenase
-
Pseudomonas sp.
1.2.1.61 4-hydroxymuconic semialdehyde dehydrogenase
-
Pseudomonas sp.
1.2.1.61 PdcG
-
Pseudomonas sp.
1.3.1.32 maleylacetate reductase
-
Pseudomonas sp.
1.3.1.32 pdcF
-
Pseudomonas sp.
1.13.11.37 PdcC
-
Pseudomonas sp.
1.13.11.66 hydroquinone 1,2-dioxygenase
-
Pseudomonas sp.
1.13.11.66 PdcDE
-
Pseudomonas sp.
1.14.13.166 p-nitrophenol monooxygenase-II
-
Pseudomonas sp.
1.14.13.166 PdcA
-
Pseudomonas sp.
1.14.13.166 PNP 4-monooxygenase
-
Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.61 50
-
-
Pseudomonas sp.
1.3.1.32 40
-
-
Pseudomonas sp.
1.13.11.66 40
-
-
Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.61 20 70
-
Pseudomonas sp.
1.3.1.32 20 70
-
Pseudomonas sp.
1.13.11.66 20 70
-
Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.61 additional information
-
purified enzyme retains 65% activity after 20 min at 60°C Pseudomonas sp.
1.3.1.32 60
-
enzyme retains 10% activity after 20 min at 60°C Pseudomonas sp.
1.13.11.66 60
-
the purified enzyme retains 35% activity after 20 min at 60°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.61 8
-
-
Pseudomonas sp.
1.3.1.32 7
-
-
Pseudomonas sp.
1.13.11.66 6
-
-
Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.61 5 10
-
Pseudomonas sp.
1.3.1.32 5 9
-
Pseudomonas sp.
1.13.11.66 3 10
-
Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.61 3 10 18% activity after 30 min at pH 3.0, and 75% activity after 30 min at pH 10.0 Pseudomonas sp.
1.3.1.32 10
-
enzyme retains 58% activity after 30 min at pH 10 Pseudomonas sp.
1.13.11.66 3
-
20% activity after 30 min at pH 3.0 Pseudomonas sp.
1.13.11.66 10
-
60% activity after 30 min at pH 10.0 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.61 NAD+
-
Pseudomonas sp.
1.3.1.32 NADH
-
Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.2.1.61 metabolism enzyme is part of the degradation pathway of 4-nitrophenol which is a priority environmental pollutant Pseudomonas sp.
1.13.11.37 metabolism the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol inthe route via 4-nitrocatechol, overview Pseudomonas sp.