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Literature summary extracted from

  • Kim, Y.; Yeom, S.; Oh, D.
    Production of beta-apo-10'-carotenal from beta-carotene by human beta-carotene-9',10'-oxygenase expressed in E. coli (2011), Biotechnol. Lett., 33, 1195-1200.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.71 synthesis production of beta-apo-10'-carotenal by recombinant enzyme. The optimum pH, temperature, detergent type, and the optimum concentrations of detergent, substrate, and enzyme for beta-apo-10'-carotenal production are 8.0, 37°C, Tween 40, 2.4%, 300 mg beta-carotene/l, and 0.25 U/ml, respectively, giving 43 mg beta-apo-10'-carotenal/l after 21 h with a conversion of 14% Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.71 expression in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.71 0.067
-
 beta-carotene pH 8.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.71 Homo sapiens Q9BYV7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.71 brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.71 beta-carotene + O2
-
 Homo sapiens 10'-apo-carotenal + beta-ionone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.71 BCO2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.71 37
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.71 35
-
 half-life 326 min Homo sapiens
1.13.11.71 40
-
 half-life 247 min Homo sapiens
1.13.11.71 45
-
 half-life 191 min Homo sapiens
1.13.11.71 50
-
 half-life 84 min Homo sapiens
1.13.11.71 55
-
 half-life 22 min Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.71 0.0053
-
 beta-carotene pH 8.0, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.71 8
-
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.71 0.079
-
 beta-carotene pH 8.0, 37°C Homo sapiens