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Literature summary extracted from

  • Kim, B.; Joe, E.; Ahn, J.
    Molecular characterization of flavonol synthase from poplar and its application to the synthesis of 3-O-methylkaempferol (2010), Biotechnol. Lett., 32, 579-584.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.20.6 2-oxoglutarate activity is completely abolished by the omission of 2-oxoglutarate from the reaction mixture Populus deltoides
1.14.20.6 ascorbate no reaction product is generated in the absence of ascorbate Populus deltoides
1.14.20.6 catalase without the addition of catalase, the enzyme activity is decreased to approximately 45% Populus deltoides

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.6 expressed with a glutathione-S-transferase tagging in Escherichia coli Populus deltoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.6 0.024
-
 (2R/3R)-dihydrokaempferol in 10 mM Tris/HCl buffer (pH 8.0), at 37°C Populus deltoides
1.14.20.6 0.031
-
 (2R/3R)-dihydroquercetin in 10 mM Tris/HCl buffer (pH 8.0), at 37°C Populus deltoides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.6 Fe2+ dependent on, the omission of Fe2+ in the reaction mixture results in 65% loss of enzyme activity Populus deltoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.20.6 38000
-
 x * 38000, SDS-PAGE Populus deltoides

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.6 Populus deltoides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.6
-
 Populus deltoides

Storage Stability

EC Number Storage Stability Organism
1.14.20.6 4°C, purified enzyme, 2 weeks, no loss of catalytic activity Populus deltoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.6 (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2
-
 Populus deltoides kaempferol + succinate + CO2 + H2O
-
 ?
1.14.20.6 (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2
-
 Populus deltoides quercetin + succinate + CO2 + H2O
-
 ?
1.14.20.6 naringenin + 2-oxoglutarate + O2
-
 Populus deltoides kaempferol + succinate + CO2 + H2O in a second reaction step with kaempferol 3-O-methyltransferase kaempferol is converted into 3-O-methylkaempferol ?

Subunits

EC Number Subunits Comment Organism
1.14.20.6 ? x * 38000, SDS-PAGE Populus deltoides

Synonyms

EC Number Synonyms Comment Organism
1.14.20.6 flavonol synthase
-
 Populus deltoides
1.14.20.6 FLS
-
 Populus deltoides