BRENDA - Enzyme Database

Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening

Park, S.H.; Park, H.Y.; Sohng, J.K.; Lee, H.C.; Liou, K.; Yoon, Y.J.; Kim, B.G.; Biotechnol. Bioeng. 102, 988-994 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.284
expressed in Escherichia coli BL21(DE3) cells
Streptomyces kanamyceticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.284
Streptomyces kanamyceticus
-
-
-
2.4.1.284
Streptomyces kanamyceticus ATCC 12853
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.284
dTDP-D-glucose + 2-deoxystreptamine
100% activity
719134
Streptomyces kanamyceticus
?
-
-
-
?
2.4.1.284
dTDP-D-glucose + 2-deoxystreptamine
100% activity
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
2.4.1.284
GDP-D-mannose + 2-deoxystreptamine
highest activity (about 200% compared to dTDP-D-glucose)
719134
Streptomyces kanamyceticus
?
-
-
-
?
2.4.1.284
GDP-D-mannose + 2-deoxystreptamine
highest activity (about 200% compared to dTDP-D-glucose)
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
2.4.1.284
additional information
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
719134
Streptomyces kanamyceticus
?
-
-
-
-
2.4.1.284
additional information
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
-
2.4.1.284
UDP-D-galactose + 2-deoxystreptamine
about 50% activity compared to dTDP-D-glucose
719134
Streptomyces kanamyceticus
?
-
-
-
?
2.4.1.284
UDP-D-galactose + 2-deoxystreptamine
about 50% activity compared to dTDP-D-glucose
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.284
expressed in Escherichia coli BL21(DE3) cells
Streptomyces kanamyceticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.284
dTDP-D-glucose + 2-deoxystreptamine
100% activity
719134
Streptomyces kanamyceticus
?
-
-
-
?
2.4.1.284
dTDP-D-glucose + 2-deoxystreptamine
100% activity
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
2.4.1.284
GDP-D-mannose + 2-deoxystreptamine
highest activity (about 200% compared to dTDP-D-glucose)
719134
Streptomyces kanamyceticus
?
-
-
-
?
2.4.1.284
GDP-D-mannose + 2-deoxystreptamine
highest activity (about 200% compared to dTDP-D-glucose)
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
2.4.1.284
additional information
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
719134
Streptomyces kanamyceticus
?
-
-
-
-
2.4.1.284
additional information
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
-
2.4.1.284
UDP-D-galactose + 2-deoxystreptamine
about 50% activity compared to dTDP-D-glucose
719134
Streptomyces kanamyceticus
?
-
-
-
?
2.4.1.284
UDP-D-galactose + 2-deoxystreptamine
about 50% activity compared to dTDP-D-glucose
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?