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A 3D localized surface plasmon resonance biosensor for the study of trivalent arsenic binding to the ArsA ATPase

Liu, C.; Balsamo, V.; Sun, D.; Naja, M.; Wang, X.; Rosen, B.; Li, C.Z.; Biosens. Bioelectron. 38, 19-26 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
7.3.2.7
gene arsA, expression of isolated ArsA ATPase in Escherichia coli strain BL21 (DE3)
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.3.2.7
additional information
-
additional information
kinetics study of ArsA-As(III) binding
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.3.2.7
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
Escherichia coli
binding of As(III) to ArsA is greatly facilitated by the presence of magnesium ion and ATP
ADP + phosphate + arsenite/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.3.2.7
Escherichia coli
-
gene arsAB in the arsRDABC operon is encoded on intracellular plasmid R773
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
binding of As(III) to ArsA is greatly facilitated by the presence of magnesium ion and ATP
719132
Escherichia coli
ADP + phosphate + arsenite/out
-
-
-
?
7.3.2.7
additional information
ArsA is the catalytic subunit of the ArsAB arsenite (As(III)) translocating ATPase, ArsA exhibits ATpase activity
719132
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
7.3.2.7
More
ArsA is the catalytic subunit of the ArsAB arsenite (As(III)) translocating ATPase, ArsA exhibits ATpase activity
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.3.2.7
gene arsA, expression of isolated ArsA ATPase in Escherichia coli strain BL21 (DE3)
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.3.2.7
additional information
-
additional information
kinetics study of ArsA-As(III) binding
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.3.2.7
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
Escherichia coli
binding of As(III) to ArsA is greatly facilitated by the presence of magnesium ion and ATP
ADP + phosphate + arsenite/out
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
binding of As(III) to ArsA is greatly facilitated by the presence of magnesium ion and ATP
719132
Escherichia coli
ADP + phosphate + arsenite/out
-
-
-
?
7.3.2.7
additional information
ArsA is the catalytic subunit of the ArsAB arsenite (As(III)) translocating ATPase, ArsA exhibits ATpase activity
719132
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.3.2.7
More
ArsA is the catalytic subunit of the ArsAB arsenite (As(III)) translocating ATPase, ArsA exhibits ATpase activity
Escherichia coli
General Information
EC Number
General Information
Commentary
Organism
7.3.2.7
physiological function
the catalytic subunit of the ArsAB arsenite (As(III)) translocating ATPase, is one of the five proteins encoded by the arsenical resistance (ars) operon of plasmid R773 in cells of Escherichia coli, that confers resistance to trivalent and pentavalent salts of the metalloid arsenic
Escherichia coli
General Information (protein specific)
EC Number
General Information
Commentary
Organism
7.3.2.7
physiological function
the catalytic subunit of the ArsAB arsenite (As(III)) translocating ATPase, is one of the five proteins encoded by the arsenical resistance (ars) operon of plasmid R773 in cells of Escherichia coli, that confers resistance to trivalent and pentavalent salts of the metalloid arsenic
Escherichia coli