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Literature summary extracted from

  • Nageshwar, Y.V.; Sheelu, G.; Shambhu, R.R.; Muluka, H.; Mehdi, N.; Malik, M.S.; Kamal, A.
    Optimization of nitrilase production from Alcaligenes faecalis MTCC 10757 (IICT-A3): effect of inducers on substrate specificity (2011), Bioprocess Biosyst. Eng., 34, 515-523.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 2,4-dichlorophenyl acetonitrile + 2 H2O Alcaligenes faecalis
-
 2,4-dichlorophenyl acetic acid + NH3
-
 ?
3.5.5.1 2-amino-4-methylbenzonitrile + 2 H2O Alcaligenes faecalis
-
 2-amino-4-methylbenzoic acid + NH3
-
 ?
3.5.5.1 2-amino-5-chloro-benzonitrile + 2 H2O Alcaligenes faecalis
-
 2-amino-5-chloro-benzoic acid + NH3
-
 ?
3.5.5.1 2-amino-6-chloro-benzonitrile + 2 H2O Alcaligenes faecalis good substrate, when the cells are induced by benzonitrile 2-amino-6-chloro-benzoic acid + NH3
-
 ?
3.5.5.1 2-aminobenzonitrile + 2 H2O Alcaligenes faecalis
-
 2-aminobenzoic acid + NH3
-
 ?
3.5.5.1 2-cyanopyridine + 2 H2O Alcaligenes faecalis
-
 pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.1 2-cyanopyridine + 2 H2O Alcaligenes faecalis MTCC 10757
-
 pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.1 3,4,5-trimethoxybenzonitrile + 2 H2O Alcaligenes faecalis
-
 3,4,5-trimethoxybenzoic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O Alcaligenes faecalis
-
 pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O Alcaligenes faecalis MTCC 10757
-
 pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.1 3-furonitrile + 2 H2O Alcaligenes faecalis
-
 furan 3-carboxylic acid + NH3
-
 ?
3.5.5.1 4,5-dimethoxybenzonitrile + 2 H2O Alcaligenes faecalis
-
 4,5-dimethoxybenzoic acid + NH3
-
 ?
3.5.5.1 4-bromobenzonitrile + 2 H2O Alcaligenes faecalis
-
 4-bromobenzoic acid + NH3
-
 ?
3.5.5.1 4-chlorobenzonitrile + 2 H2O Alcaligenes faecalis
-
 4-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O Alcaligenes faecalis
-
 pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.1 4-fluorobenzonitrile + 2 H2O Alcaligenes faecalis
-
 4-fluorobenzoic acid + NH3
-
 ?
3.5.5.1 4-methoxy-2-nitrobenzonitrile + 2 H2O Alcaligenes faecalis
-
 4-methoxy-2-nitrobenzoic acid + NH3
-
 ?
3.5.5.1 4-methoxybenzonitrile + 2 H2O Alcaligenes faecalis
-
 4-methoxybenzoic acid + NH3
-
 ?
3.5.5.1 acetonitrile + 2 H2O Alcaligenes faecalis
-
 acetic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + 2 H2O Alcaligenes faecalis preferred substrate, acrylonitrile is also a good inducer of enzyme production acrylic acid + NH3
-
 ?
3.5.5.1 adiponitrile + 2 H2O Alcaligenes faecalis
-
 adipic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O Alcaligenes faecalis preferred substrate, when the cells are induced by benzonitrile benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O Alcaligenes faecalis MTCC 10757 preferred substrate, when the cells are induced by benzonitrile benzoic acid + NH3
-
 ?
3.5.5.1 indole acetonitrile + 2 H2O Alcaligenes faecalis
-
 indole acetic acid + NH3
-
 ?
3.5.5.1 additional information Alcaligenes faecalis the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. Substrate specificity in vivo is dependent on the inducer component, overview ?
-
 ?
3.5.5.1 additional information Alcaligenes faecalis MTCC 10757 the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. Substrate specificity in vivo is dependent on the inducer component, overview ?
-
 ?
3.5.5.1 succinonitrile + 2 H2O Alcaligenes faecalis
-
 succinic acid + NH3
-
 ?
3.5.5.1 valeronitrile + 2 H2O Alcaligenes faecalis
-
 valeric acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Alcaligenes faecalis
-
 IICT-A3
-
3.5.5.1 Alcaligenes faecalis MTCC 10757
-
 IICT-A3
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 cell culture optimization of enzyme production, best conditions are glucose 1.0%, acrylonitrile 0.1%, pH 7.0 Alcaligenes faecalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 2,4-dichlorophenyl acetonitrile + 2 H2O
-
 Alcaligenes faecalis 2,4-dichlorophenyl acetic acid + NH3
-
 ?
3.5.5.1 2-amino-4-methylbenzonitrile + 2 H2O
-
 Alcaligenes faecalis 2-amino-4-methylbenzoic acid + NH3
-
 ?
3.5.5.1 2-amino-5-chloro-benzonitrile + 2 H2O
-
 Alcaligenes faecalis 2-amino-5-chloro-benzoic acid + NH3
-
 ?
3.5.5.1 2-amino-6-chloro-benzonitrile + 2 H2O good substrate, when the cells are induced by benzonitrile Alcaligenes faecalis 2-amino-6-chloro-benzoic acid + NH3
-
 ?
3.5.5.1 2-aminobenzonitrile + 2 H2O
-
 Alcaligenes faecalis 2-aminobenzoic acid + NH3
-
 ?
3.5.5.1 2-cyanopyridine + 2 H2O
-
 Alcaligenes faecalis pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.1 2-cyanopyridine + 2 H2O
-
 Alcaligenes faecalis MTCC 10757 pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.1 3,4,5-trimethoxybenzonitrile + 2 H2O
-
 Alcaligenes faecalis 3,4,5-trimethoxybenzoic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O
-
 Alcaligenes faecalis pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O
-
 Alcaligenes faecalis MTCC 10757 pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.1 3-furonitrile + 2 H2O
-
 Alcaligenes faecalis furan 3-carboxylic acid + NH3
-
 ?
3.5.5.1 4,5-dimethoxybenzonitrile + 2 H2O
-
 Alcaligenes faecalis 4,5-dimethoxybenzoic acid + NH3
-
 ?
3.5.5.1 4-bromobenzonitrile + 2 H2O
-
 Alcaligenes faecalis 4-bromobenzoic acid + NH3
-
 ?
3.5.5.1 4-chlorobenzonitrile + 2 H2O
-
 Alcaligenes faecalis 4-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O
-
 Alcaligenes faecalis pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.1 4-fluorobenzonitrile + 2 H2O
-
 Alcaligenes faecalis 4-fluorobenzoic acid + NH3
-
 ?
3.5.5.1 4-methoxy-2-nitrobenzonitrile + 2 H2O
-
 Alcaligenes faecalis 4-methoxy-2-nitrobenzoic acid + NH3
-
 ?
3.5.5.1 4-methoxybenzonitrile + 2 H2O
-
 Alcaligenes faecalis 4-methoxybenzoic acid + NH3
-
 ?
3.5.5.1 acetonitrile + 2 H2O
-
 Alcaligenes faecalis acetic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + 2 H2O preferred substrate, acrylonitrile is also a good inducer of enzyme production Alcaligenes faecalis acrylic acid + NH3
-
 ?
3.5.5.1 adiponitrile + 2 H2O
-
 Alcaligenes faecalis adipic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O preferred substrate, when the cells are induced by benzonitrile Alcaligenes faecalis benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O preferred substrate, when the cells are induced by benzonitrile Alcaligenes faecalis MTCC 10757 benzoic acid + NH3
-
 ?
3.5.5.1 indole acetonitrile + 2 H2O
-
 Alcaligenes faecalis indole acetic acid + NH3
-
 ?
3.5.5.1 additional information the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. Substrate specificity in vivo is dependent on the inducer component, overview Alcaligenes faecalis ?
-
 ?
3.5.5.1 additional information the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. No activity with any inducer on malononitrile Alcaligenes faecalis ?
-
 ?
3.5.5.1 additional information the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. Substrate specificity in vivo is dependent on the inducer component, overview Alcaligenes faecalis MTCC 10757 ?
-
 ?
3.5.5.1 additional information the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. No activity with any inducer on malononitrile Alcaligenes faecalis MTCC 10757 ?
-
 ?
3.5.5.1 succinonitrile + 2 H2O
-
 Alcaligenes faecalis succinic acid + NH3
-
 ?
3.5.5.1 valeronitrile + 2 H2O
-
 Alcaligenes faecalis valeric acid + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 35
-
-
 Alcaligenes faecalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.1 25 50 activity range, profile, overview Alcaligenes faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 50
-
 stable up to 6 h Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 8
-
-
 Alcaligenes faecalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 6 9 activity range, profile, overview Alcaligenes faecalis

Expression

EC Number Organism Comment Expression
3.5.5.1 Alcaligenes faecalis induction by epsilon-caprolactam, 3-cyanopyridine, acrylonitrile, and benzonitrile up