EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | EDTA | slightly activating at 1 mM, not at 5 mM | Alcaligenes sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.5.1 | expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pSH | Alcaligenes sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | Ag+ | - |
Alcaligenes sp. | |
3.5.5.1 | Ca2+ | - |
Alcaligenes sp. | |
3.5.5.1 | Co2+ | - |
Alcaligenes sp. | |
3.5.5.1 | Cu2+ | - |
Alcaligenes sp. | |
3.5.5.1 | EDTA | - |
Alcaligenes sp. | |
3.5.5.1 | Fe2+ | - |
Alcaligenes sp. | |
3.5.5.1 | Fe3+ | - |
Alcaligenes sp. | |
3.5.5.1 | Hg2+ | - |
Alcaligenes sp. | |
3.5.5.1 | Li+ | - |
Alcaligenes sp. | |
3.5.5.1 | Mg2+ | - |
Alcaligenes sp. | |
3.5.5.1 | Ni2+ | - |
Alcaligenes sp. | |
3.5.5.1 | Pb2+ | - |
Alcaligenes sp. | |
3.5.5.1 | Zn2+ | - |
Alcaligenes sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.1 | 21.8 | - |
(R,S)-2-hydroxy-2-phenylacetonitrile | pH 8.0, 40°C, recombinant enzyme | Alcaligenes sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | Co2+ | slightly activating at 1 mM, slightly inhibitory at 5 mM | Alcaligenes sp. | |
3.5.5.1 | Li+ | activating at 1 mM to 133%, slightly inhibitory at 5 mM | Alcaligenes sp. | |
3.5.5.1 | Mn2+ | slightly activating at 1 mM, not at 5 mM | Alcaligenes sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 376000 | - |
recombinant enzyme, gel filtration | Alcaligenes sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.1 | Alcaligenes sp. | C5IIS9 | - |
- |
3.5.5.1 | Alcaligenes sp. ECU0401 | C5IIS9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.1 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)/pSH by nickel affinity chromatography | Alcaligenes sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 19 | - |
substrate mandelonitrile, purified recombinant enzyme, pH 8.0, 40°C | Alcaligenes sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.1 | (R,S)-2-hydroxy-2-phenylacetonitrile + 2 H2O | i.e. (R,S)-mandelonitrile, preferred substrate, highly enantioselective reaction | Alcaligenes sp. | (R)-2-hydroxy-2-phenylacetic acid + NH3 | - |
? | |
3.5.5.1 | (R,S)-2-hydroxy-2-phenylacetonitrile + 2 H2O | i.e. (R,S)-mandelonitrile, preferred substrate, highly enantioselective reaction | Alcaligenes sp. ECU0401 | (R)-2-hydroxy-2-phenylacetic acid + NH3 | - |
? | |
3.5.5.1 | 2-chloromandelonitrile + 2 H2O | - |
Alcaligenes sp. | 2-chloromandelic acid + NH3 | - |
? | |
3.5.5.1 | 2-chloromandelonitrile + 2 H2O | - |
Alcaligenes sp. ECU0401 | 2-chloromandelic acid + NH3 | - |
? | |
3.5.5.1 | 2-phenylglycinonitrile + 2 H2O | - |
Alcaligenes sp. | amino(phenyl)acetic acid + NH3 | - |
? | |
3.5.5.1 | acetonitrile + 2 H2O | - |
Alcaligenes sp. | acetic acid + NH3 | - |
? | |
3.5.5.1 | acetonitrile + 2 H2O | - |
Alcaligenes sp. ECU0401 | acetic acid + NH3 | - |
? | |
3.5.5.1 | benzyl cyanide + 2 H2O | - |
Alcaligenes sp. | phenylacetic acid + NH3 | - |
? | |
3.5.5.1 | glycolonitrile + 2 H2O | - |
Alcaligenes sp. | glycolic acid + NH3 | - |
? | |
3.5.5.1 | iminodiacetonitrile + 2 H2O | - |
Alcaligenes sp. | iminodiacetic acid + 2 NH3 | - |
? | |
3.5.5.1 | iminodiacetonitrile + 2 H2O | - |
Alcaligenes sp. ECU0401 | iminodiacetic acid + 2 NH3 | - |
? | |
3.5.5.1 | additional information | substrate specificity, overview. No activity with diaminomaleonitrile, 2,3-dicyanopyrazine, 4-hydroxybenzonitrile, 2-cyano-3-phenylpropionitrile, 4-aminophthalonitrile, and 4-nitrophthalonitrile | Alcaligenes sp. | ? | - |
? | |
3.5.5.1 | additional information | substrate specificity, overview. No activity with diaminomaleonitrile, 2,3-dicyanopyrazine, 4-hydroxybenzonitrile, 2-cyano-3-phenylpropionitrile, 4-aminophthalonitrile, and 4-nitrophthalonitrile | Alcaligenes sp. ECU0401 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.5.1 | decamer | - |
Alcaligenes sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 40 | 45 | - |
Alcaligenes sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 8 | - |
- |
Alcaligenes sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 9 | - |
purified recombinant enzyme, half-life is 4 h | Alcaligenes sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.5.5.1 | Alcaligenes sp. | two-dimensional electrophoresis | - |
5.28 |