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Literature summary extracted from

  • Stocker, P.; Brunel, J.; de Rezende, L.; -do Amaral, A.; Morelli, X.; Roche, P.; Vidal, N.; Giardina, T.; Perrier, J.
    Aminoacylase 1-catalysed deacetylation of bioactives epoxides mycotoxin-derived mercapturates; 3,4-epoxyprecocenes as models of cytotoxic epoxides (2012), Biochimie, 94, 1668-1675.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 0.1
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.15
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.2
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.2
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 0.2
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.3
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.3
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 0.3
-
 N-acetyl-L-Met analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.4
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.4
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.4
-
 N-acetyl-L-Val analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.5
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 0.5
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 0.5
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 0.5
-
 N-acetyl-L-Cys analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.5
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 0.5
-
 N-acetyl-L-Val analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 1
-
 2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Homo sapiens
-
-
-
3.5.1.14 Sus scrofa P37111
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14 commercial preparation Homo sapiens
3.5.1.14 commercial preparation Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.14 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
-
 Homo sapiens S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
-
 ?
3.5.1.14 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
-
 Sus scrofa S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
-
 ?
3.5.1.14 2-acetylamino-3-((1S,2S)-2-hydroxycyclooctylsulfanyl)-propionic acid + H2O
-
 Sus scrofa S-[(1S,2S)-2-hydroxycyclooctyl]cysteine + acetate
-
 ?
3.5.1.14 2-acetylamino-3-((3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
-
 Homo sapiens S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
-
 ?
3.5.1.14 2-acetylamino-3-((3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
-
 Sus scrofa S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
-
 ?
3.5.1.14 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
-
 Homo sapiens S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
-
 ?
3.5.1.14 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
-
 Sus scrofa S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
-
 ?
3.5.1.14 N-acetyl-L-Cys + H2O
-
 Homo sapiens acetate + L-Cys
-
 ?
3.5.1.14 N-acetyl-L-Cys + H2O
-
 Sus scrofa L-Cys + acetate
-
 ?
3.5.1.14 N-acetyl-L-Met + H2O
-
 Homo sapiens acetate + L-Met
-
 ?
3.5.1.14 N-acetyl-L-Met + H2O
-
 Sus scrofa L-Met + acetate
-
 ?
3.5.1.14 N-acetyl-L-Val + H2O
-
 Sus scrofa L-Val + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 homodimer
-
 Homo sapiens
3.5.1.14 homodimer
-
 Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.5.1.14 aminoacylase 1
-
 Homo sapiens
3.5.1.14 aminoacylase 1
-
 Sus scrofa
3.5.1.14 N-acyl-L-amino acid hydrolase
-
 Homo sapiens
3.5.1.14 N-acyl-L-amino acid hydrolase
-
 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.14 1.7
-
 N-acetyl-L-Val analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 2
-
 N-acetyl-L-Val analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 3
-
 2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 9
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 18
-
 N-acetyl-L-Cys analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 19
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 19.5
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 21
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 26
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 30
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 30
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 30
-
 N-acetyl-L-Met analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 45
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 50
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 65
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 80
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 120
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 150
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.14 3
-
 2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 4
-
 N-acetyl-L-Val analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 4.3
-
 N-acetyl-L-Val analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 23
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 36
-
 N-acetyl-L-Cys analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 49
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 52
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 60
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 95
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 100
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 100
-
 N-acetyl-L-Met analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 110
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 130
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 240
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 270
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
3.5.1.14 300
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
3.5.1.14 500
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3.5.1.14 750
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens