BRENDA - Enzyme Database show

Functional and structural evaluation of cysteine residues in the human arsenic (+3 oxidation state) methyltransferase (hAS3MT)

Song, X.; Geng, Z.; Li, X.; Zhao, Q.; Hu, X.; Zhang, X.; Wang, Z.; Biochimie 93, 369-375 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21 (DE3) pLysS cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.137
C271S
the mutation does not affect the activity and structure of the enzyme
Homo sapiens
2.1.1.137
C334S
the mutation decreases the enzymatic turnover and changes the conformation of the enzyme
Homo sapiens
2.1.1.137
C360S
the mutation decreases the enzymatic turnover and changes the conformation of the enzyme
Homo sapiens
2.1.1.137
C375S
the mutation does not affect the activity and structure of the enzyme
Homo sapiens
2.1.1.137
C72S
the mutant is completely inactive
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.137
0.0016
-
arsenite
mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.00188
-
arsenite
mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.00232
-
arsenite
mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0032
-
arsenite
wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0185
-
arsenite
mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0478
-
S-adenosyl-L-methionine
wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.049
-
S-adenosyl-L-methionine
mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0495
-
S-adenosyl-L-methionine
mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0503
-
S-adenosyl-L-methionine
mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0512
-
S-adenosyl-L-methionine
mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
719012
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.1.1.137
45
-
the wild type enzyme exhibits about 40% residual activity after 120 min at 45°C
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21 (DE3) pLysS cells
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.137
C271S
the mutation does not affect the activity and structure of the enzyme
Homo sapiens
2.1.1.137
C334S
the mutation decreases the enzymatic turnover and changes the conformation of the enzyme
Homo sapiens
2.1.1.137
C360S
the mutation decreases the enzymatic turnover and changes the conformation of the enzyme
Homo sapiens
2.1.1.137
C375S
the mutation does not affect the activity and structure of the enzyme
Homo sapiens
2.1.1.137
C72S
the mutant is completely inactive
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.137
0.0016
-
arsenite
mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.00188
-
arsenite
mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.00232
-
arsenite
mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0032
-
arsenite
wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0185
-
arsenite
mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0478
-
S-adenosyl-L-methionine
wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.049
-
S-adenosyl-L-methionine
mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0495
-
S-adenosyl-L-methionine
mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0503
-
S-adenosyl-L-methionine
mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
2.1.1.137
0.0512
-
S-adenosyl-L-methionine
mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
719012
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.1.1.137
45
-
the wild type enzyme exhibits about 40% residual activity after 120 min at 45°C
Homo sapiens