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Literature summary extracted from

  • Song, X.; Geng, Z.; Li, X.; Zhao, Q.; Hu, X.; Zhang, X.; Wang, Z.
    Functional and structural evaluation of cysteine residues in the human arsenic (+3 oxidation state) methyltransferase (hAS3MT) (2011), Biochimie, 93, 369-375.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.137 expressed in Escherichia coli BL21 (DE3) pLysS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.137 C271S the mutation does not affect the activity and structure of the enzyme Homo sapiens
2.1.1.137 C334S the mutation decreases the enzymatic turnover and changes the conformation of the enzyme Homo sapiens
2.1.1.137 C360S the mutation decreases the enzymatic turnover and changes the conformation of the enzyme Homo sapiens
2.1.1.137 C375S the mutation does not affect the activity and structure of the enzyme Homo sapiens
2.1.1.137 C72S the mutant is completely inactive Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.137 0.0016
-
 arsenite mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.00188
-
 arsenite mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.00232
-
 arsenite mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.0032
-
 arsenite wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.0185
-
 arsenite mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.0478
-
 S-adenosyl-L-methionine wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.049
-
 S-adenosyl-L-methionine mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.0495
-
 S-adenosyl-L-methionine mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.0503
-
 S-adenosyl-L-methionine mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens
2.1.1.137 0.0512
-
 S-adenosyl-L-methionine mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.137 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite
-
 Homo sapiens S-adenosyl-L-homocysteine + methylarsonate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.137 arsenic (+3 oxidation state) methyltransferase
-
 Homo sapiens
2.1.1.137 AS3MT
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.137 45
-
 the wild type enzyme exhibits about 40% residual activity after 120 min at 45°C Homo sapiens