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Literature summary extracted from

  • Hou, Q.Q.; Wang, J.H.; Gao, J.; Liu, Y.J.; Liu, C.B.
    QM/MM studies on the catalytic mechanism of phenylethanolamine N-methyltransferase (2012), Biochim. Biophys. Acta, 1824, 533-541.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.28 model is prepared on the basis of X-ray crystal structure of hPNMT in complex with S-adenosyl-L-homocysteine (AdoHcy) and the inhibitor SK&F 29661 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.28 E185Q mutant has the same Km value as the wild-type hPNMT but extremely lower kcat value. E185Q mutation presents obvious structural changes in the active site Homo sapiens
2.1.1.28 E219Q mutation has little effect on the kcat value. The bridge water still forms two hydrogen bonds between Gln219 and Glu185 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.28 SK&F 29661
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.28 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.28 S-adenosyl-L-methionine + phenylethanolamine = S-adenosyl-L-homocysteine + N-methylphenylethanolamine to elucidate the detailed mechanism of enzymatic catalysis of PNMT, combined quantum-mechanical/molecular-mechanical (QM/MM) calculations are performed. The calculation results reveal that this catalysis contains three elementary steps: the deprotonation of protonated norepinphrine, the methyl transferring step and deprotonation of the methylated norepinphrine. The methyl transferring step is proved to be the rate-determining step undergoing a SN2 mechanism with an energy barrier of 16.4 kcal/mol Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.28 S-adenosyl-L-methionine + noradrenaline
-
 Homo sapiens S-adenosyl-L-homocysteine + epinephrine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.28 PNMT
-
 Homo sapiens