Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dunphy, J.T.; Schroeder, H.; Leventis, R.; Greentree, W.K; Knudsen, J.K.; Silvius, J.R.; Linder, M.E.
    Differential effects of acyl-CoA binding protein on enzymatic and non-enzymatic thioacylation of protein and peptide substrates (2000), Biochim. Biophys. Acta, 1485, 185-198.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.225 acyl-CoA binding protein i.e. ACBP strongly suppress non-enzymatic thioacylation of cysteinyl containing peptides by long-chain acyl-CoAs, usage of purified rat and recombinant bovine liver ACBP. ACBP only modestly inhibits enzymatic thioacylation of a myristoylated peptide or G-protein K-subunits under conditions where non-enzymatic thioacylation is reduced to background Bos taurus
2.3.1.225 acyl-CoA binding protein i.e. ACBP strongly suppress non-enzymatic thioacylation of cysteinyl containing peptides by long-chain acyl-CoAs, usage of purified rat and recombinant bovine liver ACBP. ACBP only modestly inhibits enzymatic thioacylation of a myristoylated peptide or G-protein K-subunits under conditions where non-enzymatic thioacylation is reduced to background Chlorocebus aethiops

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.225 acyl-CoA binding protein i.e. ACBP strongly suppress non-enzymatic thioacylation of cysteinyl containing peptides by long-chain acyl-CoAs, usage of purified rat and recombinant bovine liver ACBP. ACBP only modestly inhibits enzymatic thioacylation of a myristoylated peptide or G-protein K-subunits under conditions where non-enzymatic thioacylation is reduced to background Bos taurus
2.3.1.225 acyl-CoA binding protein i.e. ACBP strongly suppress non-enzymatic thioacylation of cysteinyl containing peptides by long-chain acyl-CoAs, usage of purified rat and recombinant bovine liver ACBP. ACBP only modestly inhibits enzymatic thioacylation of a myristoylated peptide or G-protein K-subunits under conditions where non-enzymatic thioacylation is reduced to background Chlorocebus aethiops

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.225 endoplasmic reticulum
-
Bos taurus 5783
-
2.3.1.225 endoplasmic reticulum
-
Chlorocebus aethiops 5783
-
2.3.1.225 Golgi membrane
-
Bos taurus 139
-
2.3.1.225 Golgi membrane
-
Chlorocebus aethiops 139
-
2.3.1.225 membrane
-
Bos taurus 16020
-
2.3.1.225 membrane
-
Chlorocebus aethiops 16020
-
2.3.1.225 plasma membrane
-
Bos taurus 5886
-
2.3.1.225 plasma membrane
-
Chlorocebus aethiops 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.225 palmitoyl-CoA + [G protein alpha subunit]-L-cysteine Bos taurus
-
[G protein alpha subunit]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [G protein alpha subunit]-L-cysteine Chlorocebus aethiops
-
[G protein alpha subunit]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [protein]-L-cysteine Bos taurus
-
[protein]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [protein]-L-cysteine Chlorocebus aethiops
-
[protein]-S-palmitoyl-L-cysteine + CoA
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.225 Bos taurus
-
-
-
2.3.1.225 Chlorocebus aethiops
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.225 native enzyme 120fold by anion exchange and hydroxylapatite chromatography Bos taurus
2.3.1.225 native enzyme 120fold by anion exchange and hydroxylapatite chromatography Chlorocebus aethiops

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.225 brain
-
Bos taurus
-
2.3.1.225 CV-1 cell
-
Chlorocebus aethiops
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.225 additional information PAT activity displays specificity for the acyl donor, efficiently utilizing long-chain acyl-CoAs, but not free fatty acid or S-palmitoyl-N-acetylcysteamine. PAT also shows thiolase activity Bos taurus ?
-
?
2.3.1.225 additional information PAT activity displays specificity for the acyl donor, efficiently utilizing long-chain acyl-CoAs, but not free fatty acid or S-palmitoyl-N-acetylcysteamine. PAT also shows thiolase activity Chlorocebus aethiops ?
-
?
2.3.1.225 palmitoyl-CoA + [G protein alpha subunit Gialpha1]-L-cysteine purified recombinant Gialpha1 Bos taurus [G protein alpha subunit Gialpha1]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [G protein alpha subunit Gialpha1]-L-cysteine purified recombinant Gialpha1 Chlorocebus aethiops [G protein alpha subunit Gialpha1]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [G protein alpha subunit]-L-cysteine
-
Bos taurus [G protein alpha subunit]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [G protein alpha subunit]-L-cysteine
-
Chlorocebus aethiops [G protein alpha subunit]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [protein]-L-cysteine
-
Bos taurus [protein]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [protein]-L-cysteine
-
Chlorocebus aethiops [protein]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [short lipid-modified cysteinyl-containing peptide]-L-cysteine minimum requirements for substrate recognition are a free cysteine thiol adjacent to a hydrophobic lipid anchor, either myristate or farnesyl isoprenoid, overview Bos taurus [short lipid-modified cysteinyl-containing peptide]-S-palmitoyl-L-cysteine + CoA
-
r
2.3.1.225 palmitoyl-CoA + [short lipid-modified cysteinyl-containing peptide]-L-cysteine minimum requirements for substrate recognition are a free cysteine thiol adjacent to a hydrophobic lipid anchor, either myristate or farnesyl isoprenoid, overview Chlorocebus aethiops [short lipid-modified cysteinyl-containing peptide]-S-palmitoyl-L-cysteine + CoA
-
r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.225 Pat
-
Bos taurus
2.3.1.225 Pat
-
Chlorocebus aethiops

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.225 22
-
thiolase assay at room temperature Bos taurus
2.3.1.225 22
-
thiolase assay at room temperature Chlorocebus aethiops
2.3.1.225 30 37 assay at Bos taurus
2.3.1.225 30 37 assay at Chlorocebus aethiops

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.225 6.4 8 assay at Bos taurus
2.3.1.225 6.4 8 assay at Chlorocebus aethiops
2.3.1.225 8.3
-
thiolase assay at Bos taurus
2.3.1.225 8.3
-
thiolase assay at Chlorocebus aethiops

General Information

EC Number General Information Comment Organism
2.3.1.225 physiological function reversible modification by palmitate is a feature of many signaling proteins that are associated with the cytoplasmic leaflet of the plasma membrane Bos taurus
2.3.1.225 physiological function reversible modification by palmitate is a feature of many signaling proteins that are associated with the cytoplasmic leaflet of the plasma membrane Chlorocebus aethiops