Literature summary extracted from
Meshalkina, L.E.; Solovjeva, O.N.; Khodak, Y.A.; Drutsa, V.L.; Kochetov, G.A.
Isolation and properties of human transketolase (2010), Biochemistry (Moscow), 75, 873-880.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.2.1.1 |
expression in Escherichia coli |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.2.1.1 |
additional information |
in vitro induction of the expression by arabinose decreases the amount of recombinant enzyme isolated and lowers its specific activity |
Homo sapiens |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.2.1.1 |
0.011 |
- |
D-xylulose 5-phosphate |
pH 7.6, 22°C |
Homo sapiens |
|
2.2.1.1 |
0.063 |
- |
D-ribose 5-phosphate |
pH 7.6, 22°C |
Homo sapiens |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.2.1.1 |
additional information |
presence of magnesium ions, alone or in combination with thiamine diphosphate, does not affect the activity |
Homo sapiens |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.2.1.1 |
67900 |
- |
x * 68000, SDS-PAGE, x * 67900, calculated |
Homo sapiens |
2.2.1.1 |
68000 |
- |
x * 68000, SDS-PAGE, x * 67900, calculated |
Homo sapiens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.2.1.1 |
Homo sapiens |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.2.1.1 |
recombinant protein |
Homo sapiens |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.2.1.1 |
3.64 |
- |
pH 7.6, 22°C |
Homo sapiens |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.2.1.1 |
-18°C, 50 mM glycylglycine buffer, pH 7.6, no activity loss throughout several months |
Homo sapiens |
2.2.1.1 |
4°C, 50 mM glycylglycine buffer, pH 7.6, stable for several days |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.2.1.1 |
2 D-xylulose 5-phosphate |
reversible single substrate reaction, about 6% of the double-substrate reaction rate |
Homo sapiens |
glyceraldehyde 3-phosphate + glycolaldehyde |
- |
? |
|
2.2.1.1 |
D-xylulose 5-phosphate + D-ribose 5-phosphate |
- |
Homo sapiens |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.2.1.1 |
? |
x * 68000, SDS-PAGE, x * 67900, calculated |
Homo sapiens |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.2.1.1 |
thiamine diphosphate |
Km value is 0.074 mM |
Homo sapiens |
|