BRENDA - Enzyme Database show

Identification of catalytic residues in the As(III) S-adenosylmethionine methyltransferase

Marapakala, K.; Qin, J.; Rosen, B.P.; Biochemistry 51, 944-951 (2012)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.137
glutathione
As(III) SAM methyltransferase conducts three successive rounds of oxidative methylation, with the glutathione conjugates serving as metalloid donors to the enzyme
Cyanidioschyzon sp.
Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21(DE3) cells
Cyanidioschyzon sp.
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.137
C174A
the mutation leads to loss of As(III) methylation
Cyanidioschyzon sp.
2.1.1.137
C224A
the mutation leads to loss of As(III) methylation
Cyanidioschyzon sp.
2.1.1.137
C72A
the mutation leads to loss of As(III) methylation, but still shows trivalent methylarsenate methylation
Cyanidioschyzon sp.
2.1.1.137
Y70W
the mutant exhibits metalloid binding
Cyanidioschyzon sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Cyanidioschyzon sp.
-
-
-
2.1.1.137
Cyanidioschyzon sp. 5508
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.137
Ni-NTA column chromatography
Cyanidioschyzon sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
the first product, methylarsenite, undergoes a second round of methylation faster than it dissociates from the enzyme. The second product, dimethylarsenite, dissociates faster than it undergoes the third round of methylation to the third and final product, trimethylarsine
718946
Cyanidioschyzon sp.
S-adenosyl-L-homocysteine + methylarsenite + dimethylarsenite + trimethylarsine
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
the first product, methylarsenite, undergoes a second round of methylation faster than it dissociates from the enzyme. The second product, dimethylarsenite, dissociates faster than it undergoes the third round of methylation to the third and final product, trimethylarsine
718946
Cyanidioschyzon sp. 5508
S-adenosyl-L-homocysteine + methylarsenite + dimethylarsenite + trimethylarsine
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.137
60
70
-
Cyanidioschyzon sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.137
glutathione
As(III) SAM methyltransferase conducts three successive rounds of oxidative methylation, with the glutathione conjugates serving as metalloid donors to the enzyme
Cyanidioschyzon sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21(DE3) cells
Cyanidioschyzon sp.
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.137
C174A
the mutation leads to loss of As(III) methylation
Cyanidioschyzon sp.
2.1.1.137
C224A
the mutation leads to loss of As(III) methylation
Cyanidioschyzon sp.
2.1.1.137
C72A
the mutation leads to loss of As(III) methylation, but still shows trivalent methylarsenate methylation
Cyanidioschyzon sp.
2.1.1.137
Y70W
the mutant exhibits metalloid binding
Cyanidioschyzon sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
Ni-NTA column chromatography
Cyanidioschyzon sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
the first product, methylarsenite, undergoes a second round of methylation faster than it dissociates from the enzyme. The second product, dimethylarsenite, dissociates faster than it undergoes the third round of methylation to the third and final product, trimethylarsine
718946
Cyanidioschyzon sp.
S-adenosyl-L-homocysteine + methylarsenite + dimethylarsenite + trimethylarsine
-
-
-
?
2.1.1.137
S-adenosyl-L-methionine + arsenite
the first product, methylarsenite, undergoes a second round of methylation faster than it dissociates from the enzyme. The second product, dimethylarsenite, dissociates faster than it undergoes the third round of methylation to the third and final product, trimethylarsine
718946
Cyanidioschyzon sp. 5508
S-adenosyl-L-homocysteine + methylarsenite + dimethylarsenite + trimethylarsine
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.137
60
70
-
Cyanidioschyzon sp.