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Literature summary extracted from

  • Skaff, D.A.; Ramyar, K.X.; McWhorter, W.J.; Barta, M.L.; Geisbrecht, B.V.; Miziorko, H.M.
    Biochemical and structural basis for inhibition of Enterococcus faecalis hydroxymethylglutaryl-CoA synthase, mvaS, by hymeglusin (2012), Biochemistry, 51, 4713-4722.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10 expressed in Escherichia coli BL21(DE3) cells Enterococcus faecalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.10 in complex with hymeglusin, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.3-6.5), 0.2 M NaCl, and 23% (w/v) polyethylene glycol 3350 Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 hymeglusin i.e. (2E,4E,7R)-11-[(2R,3R)-3-(hydroxymethyl)-4-oxooxetan-2-yl]-3,5,7-trimethylundeca-2,4-dienoic acid, time-dependent loss of activity is observed with more than 95% inhibition achieved at extended incubation times (10 min at 75-600 nM) Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Enterococcus faecalis Q9FD71
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10 Ni2+-NTA Sepharose column chromatography and Resource Q anion-exchange column chromatography Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Enterococcus faecalis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.10 HMG-CoA synthase
-
 Enterococcus faecalis
2.3.3.10 mvaS
-
 Enterococcus faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.7
-
 hymeglusin in 100 mM Tris-HCl (pH 8.0), at 18°C Enterococcus faecalis