Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Smith, B.C.; Anderson, M.A.; Hoadley, K.A.; Keck, J.L.; Cleland, W.W.; Denu, J.M.
    Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae (2012), Biochemistry, 51, 243-256.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.19 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.19 in complex with nicotinaldehyde, hanging drop vapor diffusion method, using 1.6 M NaOAc, 10% (w/v) ethylene glycol, and 0.1 M HEPES (pH 7.4) Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.19 C167A the mutant shows most strongly decreased kcat value compared to the wild type enzyme (below the assay detection limit) Saccharomyces cerevisiae
3.5.1.19 D51A the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae
3.5.1.19 D51N the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae
3.5.1.19 D8A the mutant shows 770fold decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae
3.5.1.19 D8E the mutant shows 100fold decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae
3.5.1.19 D8N the mutant shows 1000fold decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae
3.5.1.19 H53A the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae
3.5.1.19 H94A the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae
3.5.1.19 K122A the mutant shows 16fold decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae
3.5.1.19 K122R the mutant shows 770fold decreased kcat value compared to the wild type enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.19 3-acetylpyridine competitive inhibition Saccharomyces cerevisiae
3.5.1.19 benzaldehyde competitive inhibition Saccharomyces cerevisiae
3.5.1.19 additional information not inhibited by 10 mM EDTA Saccharomyces cerevisiae
3.5.1.19 nicotinaldehyde most potent inhibitor Saccharomyces cerevisiae
3.5.1.19 nicotinic acid competitive inhibition Saccharomyces cerevisiae
3.5.1.19 pyrazinoic acid competitive inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.19 0.0018
-
 nicotinamide Km below 0.0018 mM, mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0024
-
 nicotinamide Km below 0.0024 mM, mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0027
-
 nicotinamide Km below 0.0027 mM, mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0065
-
 nicotinamide Km below 0.0065 mM, mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0065
-
 nicotinamide mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0096
-
 nicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.025
-
 Benzamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.061
-
 5-Methylnicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.157
-
 Pyrazinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.19 Zn2+ contains tightly bound zinc. Addition of 2 mM ZnCl2 fails to increase the activity of the wild type enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Saccharomyces cerevisiae best substrate nicotinate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Saccharomyces cerevisiae P53184
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19 Ni-NTA column chromatography and Sephacryl S-300 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 1-methylnicotinamide + H2O
-
 Saccharomyces cerevisiae 1-methylnicotinate + NH3
-
 ?
3.5.1.19 5-methylnicotinamide + H2O
-
 Saccharomyces cerevisiae 5-methylnicotinate + NH3
-
 ?
3.5.1.19 benzamide + H2O
-
 Saccharomyces cerevisiae benzoate + NH3
-
 ?
3.5.1.19 additional information no activity with nicotinamide mononucleotide and NAD+ Saccharomyces cerevisiae ?
-
 ?
3.5.1.19 nicotinamide + H2O best substrate Saccharomyces cerevisiae nicotinate + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O
-
 Saccharomyces cerevisiae pyrazine-2-carboxylic acid + NH3
-
 ?
3.5.1.19 thionicotinamide + H2O
-
 Saccharomyces cerevisiae pyridine-3-carbothioic O-acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 Pnc1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.19 0.0005
-
 nicotinamide kcat below 0.0005 s-1, mutant enzyme C167A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0006
-
 nicotinamide mutant enzyme D8E, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0009
-
 nicotinamide mutant enzyme D8A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0009
-
 nicotinamide mutant enzyme D8N, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0009
-
 nicotinamide mutant enzyme K122R, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.007
-
 nicotinamide mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0088
-
 Benzamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.014
-
 nicotinamide mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.02
-
 nicotinamide mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.044
-
 nicotinamide mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.064
-
 nicotinamide mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.69
-
 nicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 1.75
-
 5-Methylnicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 2.56
-
 Pyrazinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.19 7 8
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.19 0.00094
-
 nicotinaldehyde wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0206
-
 benzaldehyde wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.12
-
 nicotinic acid wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.316
-
 3-acetylpyridine wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 6.7
-
 pyrazinoic acid wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.19 0.0086
-
 1-methylnicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.0096
-
 nicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.025
-
 Benzamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.05
-
 Thionicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.061
-
 5-Methylnicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.157
-
 Pyrazinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 0.35
-
 Benzamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 2.2
-
 nicotinamide mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 6.7
-
 nicotinamide kcat/Km above 6.7 (1/sec*mM), mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 7.4
-
 nicotinamide kcat/Km above 7.4 (1/sec*mM), mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 8.3
-
 nicotinamide kcat/Km above 8.3 (1/sec*mM), mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 16
-
 Pyrazinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 29
-
 5-Methylnicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 35
-
 nicotinamide kcat/Km above 35 (1/sec*mM), mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae
3.5.1.19 72
-
 nicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C Saccharomyces cerevisiae