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Literature summary extracted from

  • May, J.F.; Levengood, M.R.; Splain, R.A.; Brown, C.D.; Kiessling, L.L.
    A processive carbohydrate polymerase that mediates bifunctional catalysis using a single active site (2012), Biochemistry, 51, 1148-1159.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.288 D256A the mutant shows almost no activity even in the presence of Mn2+ or Mg2+ Mycobacterium tuberculosis
2.4.1.288 D256E the mutant shows almost no activity even in the presence of Mn2+ or Mg2+ Mycobacterium tuberculosis
2.4.1.288 D258A the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+ Mycobacterium tuberculosis
2.4.1.288 D258E the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme Mycobacterium tuberculosis
2.4.1.288 D371A inactive Mycobacterium tuberculosis
2.4.1.288 D372A inactive Mycobacterium tuberculosis
2.4.1.288 D372E inactive Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.288 EDTA
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.288 Mg2+ essential for catalysis Mycobacterium tuberculosis
2.4.1.288 Mn2+ essential for catalysis Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.288 Mycobacterium tuberculosis
-
-
-
2.4.1.288 Mycobacterium tuberculosis H37Rv
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 Mycobacterium tuberculosis UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 Mycobacterium tuberculosis H37Rv UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.288 GlfT2
-
 Mycobacterium tuberculosis
2.4.1.288 Rv3808c
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.4.1.288 metabolism GlfT2 catalyzes the formation of mycobacterial galactan, mediating galactan polymerization using only one active site that manifests dual regioselectivity Mycobacterium tuberculosis