Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Quartararo, C.E.; Blanchard, J.S.
    Kinetic and chemical mechanism of malate synthase from Mycobacterium tuberculosis (2011), Biochemistry, 50, 6879-6887.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.9 expressed in Escherichia coli T7 Express cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.9 C619S the mutant exhibits kinetics comparable to those of the wild type enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.9 dethio-CoA uncompetitive inhibitor versus glyoxylate and competitive inhibitor versus acetyl-CoA Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.01
-
 acetyl-CoA wild type enzyme, at pH 7.5 and 25°C Mycobacterium tuberculosis
2.3.3.9 0.03
-
 glyoxylate wild type enzyme, at pH 7.5 and 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 Mg2+ cofactor Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 80000
-
 x * 80000, His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Mycobacterium tuberculosis P9WK17
-
-
2.3.3.9 Mycobacterium tuberculosis H37Rv P9WK17
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9 Ni-NTA agarose column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O
-
 Mycobacterium tuberculosis (S)-malate + CoA
-
 ?
2.3.3.9 glyoxylate + acetyl-CoA + H2O
-
 Mycobacterium tuberculosis H37Rv (S)-malate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.9 ? x * 80000, His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.3.9 malate synthase G
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.9 23
-
 acetyl-CoA wild type enzyme, at pH 7.5 and 25°C Mycobacterium tuberculosis
2.3.3.9 23
-
 glyoxylate wild type enzyme, at pH 7.5 and 25°C Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.9 4.8 8.5
-
 Mycobacterium tuberculosis