EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.9 | expressed in Escherichia coli T7 Express cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.9 | C619S | the mutant exhibits kinetics comparable to those of the wild type enzyme | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.9 | dethio-CoA | uncompetitive inhibitor versus glyoxylate and competitive inhibitor versus acetyl-CoA | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.9 | 0.01 | - |
acetyl-CoA | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.9 | 0.03 | - |
glyoxylate | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.9 | Mg2+ | cofactor | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.9 | 80000 | - |
x * 80000, His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.9 | Mycobacterium tuberculosis | P9WK17 | - |
- |
2.3.3.9 | Mycobacterium tuberculosis H37Rv | P9WK17 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.9 | Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.9 | glyoxylate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | (S)-malate + CoA | - |
? | |
2.3.3.9 | glyoxylate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | (S)-malate + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.9 | ? | x * 80000, His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.9 | malate synthase G | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.9 | 23 | - |
acetyl-CoA | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.9 | 23 | - |
glyoxylate | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.9 | 4.8 | 8.5 | - |
Mycobacterium tuberculosis |