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Literature summary extracted from

  • Metzger, L.E.; Raetz, C.R.
    An alternative route for UDP-diacylglucosamine hydrolysis in bacterial lipid A biosynthesis (2010), Biochemistry, 49, 6715-6726.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.54 expressed in lpxh-deficient Escherichia coli CcI 21b cells Caulobacter vibrioides

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.54 EDTA complete inhibition at 2 or 0.2 mM Caulobacter vibrioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.54 0.105
-
 UDP-2,3-diacylglucosamine in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication Caulobacter vibrioides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.182 mitochondrion
-
 Arabidopsis thaliana 5739
-
2.4.1.182 additional information lipid X levels in mitochondria are 3 and 48fold higher than in chloroplasts or whole cell homogenates, respectively. Lipid X is undetectable in the plasma membrane Arabidopsis thaliana
-
-
3.6.1.54 membrane
-
 Caulobacter vibrioides 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.54 Co2+ the apparent specific activity of purified enzyme increases 6fold in the presence of added Co2+ Caulobacter vibrioides
3.6.1.54 Mg2+ the apparent specific activity of purified enzyme increases 10fold in the presence of Mg2+ versus no added metal. Enzyme activity is maximal at 0.2 mM Mg2+ and remains approximately the same up to 20 mM Mg2+ Caulobacter vibrioides
3.6.1.54 Mn2+ the apparent specific activity of purified enzyme increases 6fold in the presence of added Mn2+ Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.182 additional information Arabidopsis thaliana formation of lipid A disaccharide from UDP-GlcNAc and lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, releasing UDP. Lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, bears two R-3-hydroxymyristoyl chains in Arabidopsis thaliana and is a key precursor of lipid A. No other lipid X molecular species are detected ?
-
 ?
2.4.1.182 additional information Arabidopsis thaliana Col-0 formation of lipid A disaccharide from UDP-GlcNAc and lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, releasing UDP. Lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, bears two R-3-hydroxymyristoyl chains in Arabidopsis thaliana and is a key precursor of lipid A. No other lipid X molecular species are detected ?
-
 ?
2.4.1.182 UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate Arabidopsis thaliana
-
 UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(1->6)-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
-
 ?
2.4.1.182 UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate Arabidopsis thaliana Col-0
-
 UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(1->6)-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.6.1.54 Triton X-100 while stimulated about 3fold in the presence of 0.05% (w/v) Triton X-100, the apparent activity does not decrease at high concentrations of Triton X-100 Caulobacter vibrioides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.182 Arabidopsis thaliana F4IF99
-
-
2.4.1.182 Arabidopsis thaliana Col-0 F4IF99
-
-
3.6.1.54 Caulobacter vibrioides A0A0H3C8Q1
-
-
3.6.1.54 Caulobacter vibrioides CB15 A0A0H3C8Q1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.54 High-Trap Q Sepharose column chromatography and Superdex 200 gel filtration Caulobacter vibrioides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.54 25
-
 membane-free lysate, in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication Caulobacter vibrioides
3.6.1.54 30
-
 after 1.2fold purification, in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication Caulobacter vibrioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.182 additional information formation of lipid A disaccharide from UDP-GlcNAc and lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, releasing UDP. Lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, bears two R-3-hydroxymyristoyl chains in Arabidopsis thaliana and is a key precursor of lipid A. No other lipid X molecular species are detected Arabidopsis thaliana ?
-
 ?
2.4.1.182 additional information formation of lipid A disaccharide from UDP-GlcNAc and lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, releasing UDP. Lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, bears two R-3-hydroxymyristoyl chains in Arabidopsis thaliana and is a key precursor of lipid A. No other lipid X molecular species are detected Arabidopsis thaliana Col-0 ?
-
 ?
2.4.1.182 UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
-
 Arabidopsis thaliana UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(1->6)-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
-
 ?
2.4.1.182 UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
-
 Arabidopsis thaliana Col-0 UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(1->6)-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
-
 ?
3.6.1.54 UDP-2,3-diacylglucosamine + H2O the enzyme is selective for UDP-2,3-diacylglucosamine Caulobacter vibrioides 2,3-diacylglucosamine 1-phosphate + UMP
-
 ?
3.6.1.54 UDP-2,3-diacylglucosamine + H2O the enzyme is selective for UDP-2,3-diacylglucosamine Caulobacter vibrioides CB15 2,3-diacylglucosamine 1-phosphate + UMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.182 LpxB
-
 Arabidopsis thaliana
3.6.1.54 LpxI
-
 Caulobacter vibrioides
3.6.1.54 UDP-2,3-diacylglucosamine pyrophosphatase
-
 Caulobacter vibrioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.54 7 9
-
 Caulobacter vibrioides

General Information

EC Number General Information Comment Organism
2.4.1.182 malfunction 42fold accumulation of 2,3-diacylglucosamine 1-phosphate in Arabidopsis thaliana atlpxb-1 mutant Arabidopsis thaliana
3.6.1.54 malfunction UDP-2,3-diacylglucosamine-deficiency results in lethality Caulobacter vibrioides