EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.81 | 0.0154 | - |
acetyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 0.0886 | - |
n-propionyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 0.17 | - |
acetyl-CoA | cosubstrate neomycin B, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 0.234 | - |
malonyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.81 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.81 | acetyl-CoA + kanamycin A | - |
Pseudomonas aeruginosa | CoA + N3'-acetylkanamycin A | - |
? | |
2.3.1.81 | acetyl-CoA + neomycin B | - |
Pseudomonas aeruginosa | CoA + N3-acetylneomycin B | - |
? | |
2.3.1.81 | malonyl-CoA + kanamycin A | - |
Pseudomonas aeruginosa | CoA + N3'-malonylkanamycin A | - |
? | |
2.3.1.81 | additional information | CoASH associates with a high-affinity, catalytic site and with a secondary, low-affinity site that overlaps with the antibiotic binding pocket. The binding of CoASH to the high-affinity site occurs with a small, unfavorable enthalpy and a favorable entropy. Binding to the second site is highly exothermic and is accompanied by an unfavorable entropic contribution. The presence of an aminoglycoside alters the binding of CoASH to the enzyme dramatically such that the binding occurs with a favorable enthalpy and an unfavorable entropy. This is irrespective of which aminoglycoside is the cosubstrate and occurs without a significant change in the affinity of CoASH for the enzyme. The presence of antibiotics eliminates binding of CoASH to the second site | Pseudomonas aeruginosa | ? | - |
? | |
2.3.1.81 | n-propionyl-CoA + kanamycin A | - |
Pseudomonas aeruginosa | CoA + N3'-n-propionylkanamycin A | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.81 | 5.7 | - |
malonyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 10.2 | - |
n-propionyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 10.5 | - |
acetyl-CoA | cosubstrate neomycin B, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 39.4 | - |
acetyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.81 | 30 | - |
malonyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 120 | - |
n-propionyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 600 | - |
acetyl-CoA | cosubstrate neomycin B, pH 7.6, 25°C | Pseudomonas aeruginosa | |
2.3.1.81 | 2600 | - |
acetyl-CoA | cosubstrate kanamycin A, pH 7.6, 25°C | Pseudomonas aeruginosa |