EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.11 | gene MJ1585, expression in Escherichia coli using primersforward 5'-GGTCATATGGGGATTTTTATG-3' and reverse 5'-GCTGGATCCTTATTTCCTATCTC-3' | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.11 | methylglyoxal + D-fructose 1,6-bisphosphate | Methanocaldococcus jannaschii | - |
D-glyceraldehyde 3-phosphate + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | - |
? | |
2.2.1.11 | methylglyoxal + D-fructose 1-phosphate | Methanocaldococcus jannaschii | - |
D-glyceraldehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | - |
? | |
2.2.1.11 | additional information | Methanocaldococcus jannaschii | the enzyme catalyzes the condensation of methylglyoxal with a dihydroxyacetone-phosphate fragment, which is derived from fructose 1-phosphate and/or fructose 1,6-bisphosphate, generating 6-deoxy-5-ketofructose 1-phosphate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.11 | Methanocaldococcus jannaschii | Q58980 | gene MJ1585 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.11 | methylglyoxal + D-fructose 1,6-bisphosphate | - |
Methanocaldococcus jannaschii | D-glyceraldehyde 3-phosphate + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | - |
? | |
2.2.1.11 | methylglyoxal + D-fructose 1-phosphate | - |
Methanocaldococcus jannaschii | D-glyceraldehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | - |
? | |
2.2.1.11 | additional information | the enzyme catalyzes the condensation of methylglyoxal with a dihydroxyacetone-phosphate fragment, which is derived from fructose 1-phosphate and/or fructose 1,6-bisphosphate, generating 6-deoxy-5-ketofructose 1-phosphate | Methanocaldococcus jannaschii | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.11 | MJ1585 | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.2.1.11 | 70 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.2.1.11 | 7.5 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.2.1.11 | metabolism | the enzyme is required in the biosynthetic pathway for creating 6-deoxy-5-ketofructose 1-phosphate, DKFP, a precursor sugar for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii. This pathway is not leading via the nucleoside diphosphate mannose pathway nor a pathway involving nucleoside diphosphate derivatives of fructose 1-phosphate, fructose 2-phosphate, or fructose 1,6-bisphosphate, but methylglyoxal and a fructose 1-phosphate- or fructose 1,6-bisphosphate-derived dihydroxyacetone phosphate fragment are key intermediates in DKFP production, detailed overview. The stereochemistry of the aldolase reaction is consistent with MJ1585 also functioning as the fructose 1,6-bisphosphate aldolase, EC 4.1.2.13, used in glycolysis. The MJ1585 aldolase does not catalyze the reaction of DL-lactaldehyde with fructose 1-phosphate, fructose 1,6-phosphate, or DHAP to form 6-deoxyallulose 1-phosphate or fuculose 1-phosphate | Methanocaldococcus jannaschii |