BRENDA - Enzyme Database show

L-Aspartate semialdehyde and a 6-deoxy-5-ketohexose 1-phosphate are the precursors to the aromatic amino acids in Methanocaldococcus jannaschii

White, R.H.; Biochemistry 43, 7618-7627 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.4.1.24
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
Methanocaldococcus jannaschii
2.2.1.10
gene Mj0400, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
Methanocaldococcus jannaschii
1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.1.24
Methanocaldococcus jannaschii
P81230
-
-
1.4.1.24
Methanocaldococcus jannaschii DSM 2661
P81230
-
-
2.2.1.10
Methanocaldococcus jannaschii
Q57843
gene mj0400
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.1.24
heat precipitation and Sephadex G-25 gel filtration
Methanocaldococcus jannaschii
2.2.1.10
recombinant enzyme from Escherichia coli by heat treatment and anion exchange chromatography
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.24
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + H2O + NAD+
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate undergoes a NAD+-dependent oxidative deamination to produce 3,7-dideoxy-D-threo-hept-2,6-diulosonic acid which cyclizes to 3-dehydroquinate
718838
Methanocaldococcus jannaschii
3-dehydroquinate + NH3 + NADH + H+
-
-
-
?
1.4.1.24
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + H2O + NAD+
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate undergoes a NAD+-dependent oxidative deamination to produce 3,7-dideoxy-D-threo-hept-2,6-diulosonic acid which cyclizes to 3-dehydroquinate
718838
Methanocaldococcus jannaschii DSM 2661
3-dehydroquinate + NH3 + NADH + H+
-
-
-
?
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ
718838
Methanocaldococcus jannaschii
?
-
-
-
?
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
the MJ0400 gene product catalyzes the transaldolase reaction
718838
Methanocaldococcus jannaschii
?
-
-
-
?
2.2.1.10
additional information
GC-MS analysis of reaction intermediates, overview. The enzyme is active as aldose with erythrose 4-phosphate and pyruvate producing a different stereoisomer from 3-deoxy-D-arabino-2-heptulosonate-7-phosphate
718838
Methanocaldococcus jannaschii
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.2.1.10
60
-
assay at
Methanocaldococcus jannaschii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.10
7.2
-
assay at
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.1.24
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
Methanocaldococcus jannaschii
2.2.1.10
gene Mj0400, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
Methanocaldococcus jannaschii
1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.1.24
heat precipitation and Sephadex G-25 gel filtration
Methanocaldococcus jannaschii
2.2.1.10
recombinant enzyme from Escherichia coli by heat treatment and anion exchange chromatography
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.24
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + H2O + NAD+
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate undergoes a NAD+-dependent oxidative deamination to produce 3,7-dideoxy-D-threo-hept-2,6-diulosonic acid which cyclizes to 3-dehydroquinate
718838
Methanocaldococcus jannaschii
3-dehydroquinate + NH3 + NADH + H+
-
-
-
?
1.4.1.24
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + H2O + NAD+
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate undergoes a NAD+-dependent oxidative deamination to produce 3,7-dideoxy-D-threo-hept-2,6-diulosonic acid which cyclizes to 3-dehydroquinate
718838
Methanocaldococcus jannaschii DSM 2661
3-dehydroquinate + NH3 + NADH + H+
-
-
-
?
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ
718838
Methanocaldococcus jannaschii
?
-
-
-
?
2.2.1.10
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
the MJ0400 gene product catalyzes the transaldolase reaction
718838
Methanocaldococcus jannaschii
?
-
-
-
?
2.2.1.10
additional information
GC-MS analysis of reaction intermediates, overview. The enzyme is active as aldose with erythrose 4-phosphate and pyruvate producing a different stereoisomer from 3-deoxy-D-arabino-2-heptulosonate-7-phosphate
718838
Methanocaldococcus jannaschii
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.2.1.10
60
-
assay at
Methanocaldococcus jannaschii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.2.1.10
7.2
-
assay at
Methanocaldococcus jannaschii
General Information
EC Number
General Information
Commentary
Organism
2.2.1.10
metabolism
Mj0400 is involved in the biosynthesis of aromatic compounds, including 3-dehydroquinate via an undetermined intermediate and 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, which is formed by NAD+-dependent Mj1249 from the intermediate compound. Also 4,5-dihydroxy-6-methylpipecolinic acid is formed from the intermediate. Pathway analysis, detailed overview
Methanocaldococcus jannaschii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.2.1.10
metabolism
Mj0400 is involved in the biosynthesis of aromatic compounds, including 3-dehydroquinate via an undetermined intermediate and 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, which is formed by NAD+-dependent Mj1249 from the intermediate compound. Also 4,5-dihydroxy-6-methylpipecolinic acid is formed from the intermediate. Pathway analysis, detailed overview
Methanocaldococcus jannaschii