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Literature summary extracted from

  • White, R.H.
    L-Aspartate semialdehyde and a 6-deoxy-5-ketohexose 1-phosphate are the precursors to the aromatic amino acids in Methanocaldococcus jannaschii (2004), Biochemistry, 43, 7618-7627.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.24 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Methanocaldococcus jannaschii
2.2.1.10 gene Mj0400, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.10 L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate Methanocaldococcus jannaschii 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.24 Methanocaldococcus jannaschii P81230
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1.4.1.24 Methanocaldococcus jannaschii DSM 2661 P81230
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2.2.1.10 Methanocaldococcus jannaschii Q57843 gene mj0400
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.24 heat precipitation and Sephadex G-25 gel filtration Methanocaldococcus jannaschii
2.2.1.10 recombinant enzyme from Escherichia coli by heat treatment and anion exchange chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.24 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + H2O + NAD+ 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate undergoes a NAD+-dependent oxidative deamination to produce 3,7-dideoxy-D-threo-hept-2,6-diulosonic acid which cyclizes to 3-dehydroquinate Methanocaldococcus jannaschii 3-dehydroquinate + NH3 + NADH + H+
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 ?
1.4.1.24 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + H2O + NAD+ 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate undergoes a NAD+-dependent oxidative deamination to produce 3,7-dideoxy-D-threo-hept-2,6-diulosonic acid which cyclizes to 3-dehydroquinate Methanocaldococcus jannaschii DSM 2661 3-dehydroquinate + NH3 + NADH + H+
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 ?
2.2.1.10 L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ Methanocaldococcus jannaschii ?
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 ?
2.2.1.10 L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate the MJ0400 gene product catalyzes the transaldolase reaction Methanocaldococcus jannaschii ?
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 ?
2.2.1.10 additional information GC-MS analysis of reaction intermediates, overview. The enzyme is active as aldose with erythrose 4-phosphate and pyruvate producing a different stereoisomer from 3-deoxy-D-arabino-2-heptulosonate-7-phosphate Methanocaldococcus jannaschii ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.24 MJ1249 protein
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 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.10 60
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 assay at Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.10 7.2
-
 assay at Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.2.1.10 metabolism Mj0400 is involved in the biosynthesis of aromatic compounds, including 3-dehydroquinate via an undetermined intermediate and 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, which is formed by NAD+-dependent Mj1249 from the intermediate compound. Also 4,5-dihydroxy-6-methylpipecolinic acid is formed from the intermediate. Pathway analysis, detailed overview Methanocaldococcus jannaschii