BRENDA - Enzyme Database show

Alternative splicing variants of human arsenic (+3 oxidation state) methyltransferase

Sumi, D.; Fukushima, K.; Miyataka, H.; Himeno, S.; Biochem. Biophys. Res. Commun. 415, 48-53 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21(DE3)pLysS cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.137
additional information
an exon-4 and -5 skipping (DELTA4,5) truncated mutant form does not convert arsenite to monomethylarsonate or dimethylarsinic acid
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
31100
-
x * 31100, truncated exon-4 and -5 skipping (DELTA4,5) mutant form, calculated from amino acid sequence
Homo sapiens
2.1.1.137
41700
-
x * 41700, full-lenth wild type form, calculated from amino acid sequence
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.137
Ni Sepharose column chromatography, gel filtration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.137
A-549 cell
-
Homo sapiens
-
2.1.1.137
HEK-293 cell
-
Homo sapiens
-
2.1.1.137
Hep-G2 cell
-
Homo sapiens
-
2.1.1.137
HL-60 cell
-
Homo sapiens
-
2.1.1.137
K-562 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
718748
Homo sapiens
S-adenosyl-L-homocysteine + monomethylarsonate + dimethylarsinic acid
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 31100, truncated exon-4 and -5 skipping (DELTA4,5) mutant form, calculated from amino acid sequence; x * 41700, full-lenth wild type form, calculated from amino acid sequence
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
expressed in Escherichia coli BL21(DE3)pLysS cells
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.137
additional information
an exon-4 and -5 skipping (DELTA4,5) truncated mutant form does not convert arsenite to monomethylarsonate or dimethylarsinic acid
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.137
31100
-
x * 31100, truncated exon-4 and -5 skipping (DELTA4,5) mutant form, calculated from amino acid sequence
Homo sapiens
2.1.1.137
41700
-
x * 41700, full-lenth wild type form, calculated from amino acid sequence
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.137
Ni Sepharose column chromatography, gel filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.137
A-549 cell
-
Homo sapiens
-
2.1.1.137
HEK-293 cell
-
Homo sapiens
-
2.1.1.137
Hep-G2 cell
-
Homo sapiens
-
2.1.1.137
HL-60 cell
-
Homo sapiens
-
2.1.1.137
K-562 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
718748
Homo sapiens
S-adenosyl-L-homocysteine + monomethylarsonate + dimethylarsinic acid
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.137
?
x * 31100, truncated exon-4 and -5 skipping (DELTA4,5) mutant form, calculated from amino acid sequence; x * 41700, full-lenth wild type form, calculated from amino acid sequence
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
2.1.1.137
physiological function
the enzyme plays an important role in the detoxification of arsenicals
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.137
physiological function
the enzyme plays an important role in the detoxification of arsenicals
Homo sapiens