Literature summary extracted from

Chen, Y.; Rosazza, J.
A bacterial nitric oxide synthase from a Nocardia species (1994), Biochem. Biophys. Res. Commun., 203, 1251-1258.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.14.14.47	Ngamma-nitro-L-arginine	competitive	Nocardia sp.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.14.47	0.0082	-	L-arginine	pH 7.5, 25°C	Nocardia sp.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.14.14.47	soluble	-	Nocardia sp.	-	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.47	Nocardia sp.	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.14.47	partial	Nocardia sp.

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.14.14.47	318	-	pH 7.5, 25°C	Nocardia sp.

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.47	2 L-arginine + 3 reduced flavodoxin + 4 O2	-	Nocardia sp.	2 L-citrulline + 2 nitric oxide + 3 oxidized flavodoxin + 4 H2O	-	?

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.14.47	FAD	-	Nocardia sp.
1.14.14.47	FMN	-	Nocardia sp.
1.14.14.47	NADPH	-	Nocardia sp.
1.14.14.47	tetrahydrobiopterin	-	Nocardia sp.

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.14.14.47	0.0146	-	Ngamma-nitro-L-arginine	pH 7.5, 25°C	Nocardia sp.

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Release 2023.1 (January 2023)