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Literature summary extracted from

  • Zhou, Y.; Xin, Y.; Sha, S.; Ma, Y.
    Kinetic properties of Mycobacterium tuberculosis bifunctional GlmU (2011), Arch. Microbiol., 193, 751-757.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.157 drug development GlmU is a target for drug development against Mycobacteria Mycobacterium tuberculosis
2.7.7.23 analysis quick and sensitive enzyme detection assay based on the diphosphatase-coupled colorimetric method with malachite green, and assay for glucosamine-1-phosphate N-acetyltransferase activity of the bifunctional enzyme, both applicable in microtitter plates Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.157 additional information
-
 additional information steady-state kinetics and kinetic analysis of the acetyltransferase activity of GlmU, overview Mycobacterium tuberculosis
2.3.1.157 0.061
-
 alpha-D-glucosamine 1-phosphate pH 7.5, 37°C Mycobacterium tuberculosis
2.3.1.157 0.224
-
 acetyl-CoA pH 7.5, 37°C Mycobacterium tuberculosis
2.7.7.23 0.024
-
 UTP pH 7.5, 37°C Mycobacterium tuberculosis
2.7.7.23 0.044
-
 N-acetyl-D-glucosamine 1-phosphate pH 7.5, 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.157 Mg2+ has no effect on the activity of GlmU acetyltransferase up to 50 mM Mycobacterium tuberculosis
2.7.7.23 Mg2+ absolutely required, optimal concentration 20 mM Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 additional information Mycobacterium tuberculosis GlmU also catalyzes the reaction of the N-acetylglucosamine-1-phosphate uridyltransferase, EC 2.7.7.23, catalyzing the synthesis of UDP-GlcNAc from GlcNAc-1-phosphate and UTP ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.157 Mycobacterium tuberculosis
-
-
-
2.7.7.23 Mycobacterium tuberculosis P9WMN3 bifunctional UDP-N-acetylglucosamine diphosphorylase, EC 2.7.7.23, and glucosamine-1-phosphate N-acetyltransferase, EC 2.3.1.157
-
2.7.7.23 Mycobacterium tuberculosis H37Rv P9WMN3 bifunctional UDP-N-acetylglucosamine diphosphorylase, EC 2.7.7.23, and glucosamine-1-phosphate N-acetyltransferase, EC 2.3.1.157
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Mycobacterium tuberculosis CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 additional information GlmU also catalyzes the reaction of the N-acetylglucosamine-1-phosphate uridyltransferase, EC 2.7.7.23, catalyzing the synthesis of UDP-GlcNAc from GlcNAc-1-phosphate and UTP Mycobacterium tuberculosis ?
-
 ?
2.7.7.23 N-acetyl-D-glucosamine 1-phosphate + UTP
-
 Mycobacterium tuberculosis UDP-N-acetyl-D-glucosamine + diphosphate
-
 ?
2.7.7.23 N-acetyl-D-glucosamine 1-phosphate + UTP
-
 Mycobacterium tuberculosis H37Rv UDP-N-acetyl-D-glucosamine + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.157 GlmU
-
 Mycobacterium tuberculosis
2.3.1.157 glucosamine-1-phosphate acetyltransferase
-
 Mycobacterium tuberculosis
2.7.7.23 GlmU
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.157 30
-
-
 Mycobacterium tuberculosis
2.7.7.23 40
-
-
 Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.157 16 80 activity range Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.157 82.47
-
 acetyl-CoA pH 7.5, 25°C Mycobacterium tuberculosis
2.3.1.157 82.47
-
 alpha-D-glucosamine 1-phosphate pH 7.5, 25°C Mycobacterium tuberculosis
2.7.7.23 3.04
-
 UTP pH 7.5, 37°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.157 8
-
-
 Mycobacterium tuberculosis
2.7.7.23 8
-
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.157 7 11 activity range Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.3.1.157 physiological function essentiality of GlmU to mycobacterial survival Mycobacterium tuberculosis