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Literature summary extracted from

  • Ishikawa, R.; Ishido, Y.; Tachikawa, A.; Kawasaki, H.; Matsuzawa, H.; Wakagi, T.
    Aeropyrum pernix K1, a strictly aerobic and hyperthermophilic archaeon, has two terminal oxidases, cytochrome ba3 and cytochrome aa3 (2002), Arch. Microbiol., 179, 42-49.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.4 ferrocytochrome c 0.002 mM, 50% inhibition of N,N,N',N'-tetramethyl-1,4-phenylenediamine-ascorbate dependent oxygen consumption Aeropyrum pernix
7.1.1.4 KCN 1 mM, 76% inhibition Aeropyrum pernix
7.1.1.4 NaN3 1 mM, 76% inhibition Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.4 0.032
-
O2 pH 7.5, 25°C Aeropyrum pernix
7.1.1.4 0.333
-
N,N,N',N'-tetramethyl-1,4-phenylenediamine pH 7.5, 25°C Aeropyrum pernix

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.4 membrane
-
Aeropyrum pernix 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.4 11000
-
x * 47000 + x * 19000 + x * 11000, the enzyme is composed of at least three polypeptides, SDS-PAGE Aeropyrum pernix
7.1.1.4 19000
-
x * 47000 + x * 19000 + x * 11000, the enzyme is composed of at least three polypeptides, SDS-PAGE Aeropyrum pernix
7.1.1.4 47000
-
x * 47000 + x * 19000 + x * 11000, the enzyme is composed of at least three polypeptides, SDS-PAGE Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.4 Aeropyrum pernix
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.4
-
Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.4 4 ferrocytochrome c + O2 + 4 H+
-
Aeropyrum pernix 4 ferricytochrome c + 2 H2O
-
?
7.1.1.4 N,N,N',N'-tetramethyl-1,4-phenylenediamine + O2 N,N,N',N'-tetramethyl-1,4-phenylenediamine-ascorbate dependent oxygen consumption Aeropyrum pernix ?
-
?

Subunits

EC Number Subunits Comment Organism
7.1.1.4 ? x * 47000 + x * 19000 + x * 11000, the enzyme is composed of at least three polypeptides, SDS-PAGE Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
7.1.1.4 cytochrome oxidase CO-2
-
Aeropyrum pernix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.4 1.5
-
ferrocytochrome c pH 7.5, 40°C Aeropyrum pernix
7.1.1.4 66.9
-
N,N,N',N'-tetramethyl-1,4-phenylenediamine pH 7.5, 25°C Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.4 7.4
-
-
Aeropyrum pernix