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Literature summary extracted from

  • Lueddeke, F.; Wuelfing, A.; Timke, M.; Germer, F.; Weber, J.; Dikfidan, A.; Rahnfeld, T.; Linder, D.; Meyerdierks, A.; Harder, J.
    Geraniol and geranial dehydrogenases induced in anaerobic monoterpene degradation by Castellaniella defragrans (2012), Appl. Environ. Microbiol., 78, 2128-2136.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.347 expressed in Escherichia coli strain BL21 Star(DE3) Castellaniella defragrans
1.1.1.347 expression in Escherichia coli Castellaniella defragrans
1.2.1.86 expressed in Escherichia coli BL21 Star(DE3) cells Castellaniella defragrans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.347 1-cyclohexyl-N-(2-morpholinoethyl)carbodiimide I80 of less than 34 mM Castellaniella defragrans
1.1.1.347 diethylpyrocarbonate I80 of less than 17 mM Castellaniella defragrans
1.1.1.347 EDTA 7 mM, 96% inhibition; 7 mM EDTA inhibits the enzyme by 96% Castellaniella defragrans
1.1.1.347 N-ethylmaleimide I80 of less than 0.5 mM Castellaniella defragrans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.347 0.0033
-
 geraniol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.0044
-
 (S)-perillyl alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.005
-
 geraniol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 0.006
-
 cumic alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.007
-
 (S)-perillyl alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.007
-
 (S)-perillyl alcohol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 0.021
-
 cumic alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.021
-
 p-cumic alcohol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 0.0232
-
 nerol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.045
-
 nerol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.045
-
 nerol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 0.05
-
 geraniol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.0575
-
 citronellol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.086
-
 citronellol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.086
-
 citronellol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 0.1157
-
 benzyl alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.17
-
 benzyl alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 0.17
-
 benzyl alcohol pH 9.4, 21°C Castellaniella defragrans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.347 Zn2+ contains zinc Castellaniella defragrans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.347 38272
-
 2 * 38272, calculated from amino acid sequence Castellaniella defragrans
1.1.1.347 38272
-
 2 * 38272, calculated Castellaniella defragrans
1.1.1.347 39000
-
 2 * 39000, SDS-PAGE Castellaniella defragrans
1.1.1.347 52000
-
 gel filtration Castellaniella defragrans
1.1.1.347 52000 91000 gel filtration Castellaniella defragrans
1.1.1.347 85000
-
 PAGE Castellaniella defragrans
1.2.1.86 53000
-
 x * 53000, SDS-PAGE Castellaniella defragrans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.347 geraniol + NAD+ Castellaniella defragrans
-
 geranial + NADH + H+
-
 ?
1.1.1.347 geraniol + NAD+ Castellaniella defragrans 65Phen
-
 geranial + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.347 Castellaniella defragrans H1ZV38
-
-
1.1.1.347 Castellaniella defragrans 65Phen H1ZV38
-
-
1.2.1.86 Castellaniella defragrans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.347 DEAE column chromatography and phenyl-Sepharose 6 gel filtration Castellaniella defragrans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.347 additional information
-
 the crude cell extract shows a specific activity of 0.056 units/mg, while the 114fold purified enzyme shows a specific activity of 4.33 units/mg at pH 9.4 and 21°C Castellaniella defragrans
1.1.1.347 4.33
-
 pH 9.4, 21°C Castellaniella defragrans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.347 (S)-perillyl alcohol + NAD+ most efficient substrate Castellaniella defragrans ? + NADH + H+
-
 ?
1.1.1.347 (S)-perillyl alcohol + NAD+
-
 Castellaniella defragrans perillyl aldehyde + NADH
-
 ?
1.1.1.347 benzyl alcohol + NAD+
-
 Castellaniella defragrans benzaldehyde + NADH + H+
-
 ?
1.1.1.347 benzyl alcohol + NAD+
-
 Castellaniella defragrans 65Phen benzaldehyde + NADH + H+
-
 ?
1.1.1.347 benzyl alcohol + NAD+
-
 Castellaniella defragrans ?
-
 ?
1.1.1.347 citronellol + NAD+
-
 Castellaniella defragrans ?
-
 ?
1.1.1.347 citronellol + NAD+
-
 Castellaniella defragrans citronellal + NADH + H+
-
 ?
1.1.1.347 cumic alcohol + NAD+
-
 Castellaniella defragrans ?
-
 ?
1.1.1.347 geraniol + dichlorophenolindophenol
-
 Castellaniella defragrans ?
-
 ?
1.1.1.347 geraniol + NAD+
-
 Castellaniella defragrans geranial + NADH + H+
-
 ?
1.1.1.347 geraniol + NAD+
-
 Castellaniella defragrans 65Phen geranial + NADH + H+
-
 ?
1.1.1.347 geraniol + oxidized dichlorophenolindophenol
-
 Castellaniella defragrans geranial + reduced dichlorophenolindophenol
-
 ?
1.1.1.347 additional information the enzyme cannot use phenazine methosulfate as alternative acceptor Castellaniella defragrans ?
-
 ?
1.1.1.347 additional information phenazine methosulfate is not accepted as an electron acceptor Castellaniella defragrans ?
-
 ?
1.1.1.347 additional information the enzyme cannot use phenazine methosulfate as alternative acceptor Castellaniella defragrans 65Phen ?
-
 ?
1.1.1.347 additional information phenazine methosulfate is not accepted as an electron acceptor Castellaniella defragrans 65Phen ?
-
 ?
1.1.1.347 nerol + NAD+
-
 Castellaniella defragrans neral + NADH + H+
-
 ?
1.1.1.347 nerol + NAD+
-
 Castellaniella defragrans 65Phen neral + NADH + H+
-
 ?
1.1.1.347 nerol + NAD+
-
 Castellaniella defragrans ?
-
 ?
1.1.1.347 p-cumic alcohol + NAD+
-
 Castellaniella defragrans ? + NADH + H+
-
 ?
1.2.1.86 geranial + H2O + NAD+
-
 Castellaniella defragrans geranate + NADH + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.347 dimer 2 * 38272, calculated Castellaniella defragrans
1.1.1.347 homodimer 2 * 39000, SDS-PAGE Castellaniella defragrans
1.1.1.347 homodimer 2 * 38272, calculated from amino acid sequence Castellaniella defragrans
1.2.1.86 ? x * 53000, SDS-PAGE Castellaniella defragrans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.347 GeDH
-
 Castellaniella defragrans
1.1.1.347 geoA
-
 Castellaniella defragrans
1.1.1.347 geraniol DH
-
 Castellaniella defragrans
1.2.1.86 GADH
-
 Castellaniella defragrans
1.2.1.86 geoB
-
 Castellaniella defragrans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.347 9.4
-
-
 Castellaniella defragrans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.347 additional information NADPH is ineffective as a cosubstrate Castellaniella defragrans
1.1.1.347 NAD+ dependent on, no activity with NADP+ Castellaniella defragrans
1.1.1.347 NADH enzyme reduces one molecule of NAD+ to NADH per geraniol molecule provided Castellaniella defragrans
1.2.1.86 additional information enzyme activity is independent of coenzyme A Castellaniella defragrans

Expression

EC Number Organism Comment Expression
1.1.1.347 Castellaniella defragrans enzyme activity is induced during growth with limonene in comparison to growth with acetate up
1.1.1.347 Castellaniella defragrans activitiy is induced during growth with limonene in comparison to growth with acetate up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.347 50
-
 citronellol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 100
-
 citronellol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 100
-
 citronellol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 110
-
 benzyl alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 220
-
 benzyl alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 220
-
 benzyl alcohol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 310
-
 nerol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 310
-
 nerol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 310
-
 nerol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 520
-
 cumic alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 520
-
 p-cumic alcohol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 620
-
 geraniol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 940
-
 cumic alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 1570
-
 geraniol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 1570
-
 geraniol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 2020
-
 (S)-perillyl alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1.1.1.347 2020
-
 (S)-perillyl alcohol pH 9.4, 21°C Castellaniella defragrans
1.1.1.347 3550
-
 (S)-perillyl alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans