EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.95 | gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Arachis hypogaea |
2.3.1.95 | gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Pinus densiflora |
2.3.1.95 | gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Vitis vinifera |
2.3.1.95 | gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Pinus strobus |
2.3.1.95 | gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Polygonum cuspidatum |
2.3.1.95 | gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Pinus massoniana |
2.3.1.95 | gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Psilotum nudum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.95 | acetyl-CoA | competitive inhibition | Arachis hypogaea | |
2.3.1.95 | acetyl-CoA | - |
Pinus massoniana | |
2.3.1.95 | acetyl-CoA | competitive inhibition | Pinus strobus | |
2.3.1.95 | acetyl-CoA | competitive inhibition | Vitis vinifera |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.95 | 0.00287 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus strobus | |
2.3.1.95 | 0.00443 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Arachis hypogaea | |
2.3.1.95 | 0.00856 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Vitis vinifera | |
2.3.1.95 | 0.0119 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus massoniana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | Arachis hypogaea | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | Pinus densiflora | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | Vitis vinifera | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | Pinus strobus | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | Polygonum cuspidatum | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | Pinus massoniana | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | Psilotum nudum | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.95 | Arachis hypogaea | - |
gene sts | - |
2.3.1.95 | Pinus densiflora | - |
gene sts | - |
2.3.1.95 | Pinus massoniana | - |
gene sts | - |
2.3.1.95 | Pinus strobus | - |
gene sts | - |
2.3.1.95 | Polygonum cuspidatum | - |
gene sts | - |
2.3.1.95 | Psilotum nudum | - |
gene sts | - |
2.3.1.95 | Vitis vinifera | - |
gene sts | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.95 | recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Arachis hypogaea |
2.3.1.95 | recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Pinus densiflora |
2.3.1.95 | recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Vitis vinifera |
2.3.1.95 | recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Pinus strobus |
2.3.1.95 | recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Polygonum cuspidatum |
2.3.1.95 | recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Pinus massoniana |
2.3.1.95 | recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Psilotum nudum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Arachis hypogaea | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Pinus densiflora | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Vitis vinifera | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Pinus strobus | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Polygonum cuspidatum | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Pinus massoniana | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
2.3.1.95 | 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Psilotum nudum | 4 CoA + trans-resveratrol + 4 CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.95 | stilbene synthase | - |
Arachis hypogaea |
2.3.1.95 | stilbene synthase | - |
Pinus densiflora |
2.3.1.95 | stilbene synthase | - |
Vitis vinifera |
2.3.1.95 | stilbene synthase | - |
Pinus strobus |
2.3.1.95 | stilbene synthase | - |
Polygonum cuspidatum |
2.3.1.95 | stilbene synthase | - |
Pinus massoniana |
2.3.1.95 | stilbene synthase | - |
Psilotum nudum |
2.3.1.95 | STS | - |
Arachis hypogaea |
2.3.1.95 | STS | - |
Pinus densiflora |
2.3.1.95 | STS | - |
Vitis vinifera |
2.3.1.95 | STS | - |
Pinus strobus |
2.3.1.95 | STS | - |
Polygonum cuspidatum |
2.3.1.95 | STS | - |
Pinus massoniana |
2.3.1.95 | STS | - |
Psilotum nudum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.95 | 30 | - |
assay at | Arachis hypogaea |
2.3.1.95 | 30 | - |
assay at | Pinus densiflora |
2.3.1.95 | 30 | - |
assay at | Vitis vinifera |
2.3.1.95 | 30 | - |
assay at | Pinus strobus |
2.3.1.95 | 30 | - |
assay at | Polygonum cuspidatum |
2.3.1.95 | 30 | - |
assay at | Pinus massoniana |
2.3.1.95 | 30 | - |
assay at | Psilotum nudum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.95 | 0.0005 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus strobus | |
2.3.1.95 | 0.0017 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Arachis hypogaea | |
2.3.1.95 | 0.0023 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus massoniana | |
2.3.1.95 | 0.003 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Vitis vinifera |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.95 | 4 | - |
assay at | Arachis hypogaea |
2.3.1.95 | 4 | - |
assay at | Pinus densiflora |
2.3.1.95 | 4 | - |
assay at | Vitis vinifera |
2.3.1.95 | 4 | - |
assay at | Pinus strobus |
2.3.1.95 | 4 | - |
assay at | Polygonum cuspidatum |
2.3.1.95 | 4 | - |
assay at | Pinus massoniana |
2.3.1.95 | 4 | - |
assay at | Psilotum nudum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.95 | 0.02 | - |
acetyl-CoA | pH 4.0, 30°C, uncompetitive inhibition | Vitis vinifera | |
2.3.1.95 | 0.04 | - |
acetyl-CoA | pH 4.0, 30°C, uncompetitive inhibition | Pinus massoniana | |
2.3.1.95 | 0.07 | - |
acetyl-CoA | pH 4.0, 30°C, recombinant STS, uncompetitive inhibition | Arachis hypogaea | |
2.3.1.95 | 0.15 | - |
acetyl-CoA | pH 4.0, 30°C, uncompetitive inhibition | Pinus strobus | |
2.3.1.95 | 0.46 | - |
acetyl-CoA | pH 4.0, 30°C, competitive inhibition | Vitis vinifera | |
2.3.1.95 | 0.52 | - |
acetyl-CoA | pH 4.0, 30°C, recombinant STS, competitive inhibition | Arachis hypogaea | |
2.3.1.95 | 0.787 | - |
acetyl-CoA | pH 4.0, 30°C, competitive inhibition | Pinus massoniana | |
2.3.1.95 | 0.97 | - |
acetyl-CoA | pH 4.0, 30°C, competitive inhibition | Pinus strobus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.95 | 0.107 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus massoniana | |
2.3.1.95 | 0.16 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus strobus | |
2.3.1.95 | 0.21 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Vitis vinifera | |
2.3.1.95 | 0.314 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Arachis hypogaea |