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Literature summary extracted from

  • Mihasan, M.; Chiribau, C.B.; Friedrich, T.; Artenie, V.; Brandsch, R.
    An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism (2007), Appl. Environ. Microbiol., 73, 2479-2485.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.328 expression in Escherichia coli Paenarthrobacter nicotinovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.328 0.0062
-
 1,4-Naphthoquinone pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.1.1.328 0.0088
-
 1,4-benzoquinone pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.1.1.328 0.023
-
 oxidized 2,6-dichlorophenol-indophenol pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.1.1.328 0.035
-
 NADH pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.328 Paenarthrobacter nicotinovorans
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.328 0.004
-
 substrate duraquinone, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.1.1.328 0.102
-
 substrate menadione, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.1.1.328 0.368
-
 substrate 2,6-dichlorophenol-indophenol, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.1.1.328 0.536
-
 substrate 1,4-benzoquinone, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.1.1.328 0.733
-
 substrate 1,4-naphthoquinone, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.1.1.328 0.893
-
 substrate nicotine blue, pH 7.1, temperature not specified in the publication Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.328 1,4-benzoquinone + NADPH + H+
-
 Paenarthrobacter nicotinovorans 1,4-benzoquinol + NADP+
-
 ?
1.1.1.328 1,4-naphthoquinone + NADPH + H+
-
 Paenarthrobacter nicotinovorans 1,4-naphthoquinol + NADP+
-
 ?
1.1.1.328 4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+
-
 Paenarthrobacter nicotinovorans ? + NADP+
-
 ?
1.1.1.328 duroquinone + NADPH + H+
-
 Paenarthrobacter nicotinovorans duroquinol + NADP+
-
 ?
1.1.1.328 menadione + NADPH + H+
-
 Paenarthrobacter nicotinovorans menadiol + NADP+
-
 ?
1.1.1.328 nicotine blue + NADH + H+
-
 Paenarthrobacter nicotinovorans nicotine blue leuco form + NAD+ two-electron transfer reaction ?
1.1.1.328 nicotine blue + NADPH + H+
-
 Paenarthrobacter nicotinovorans nicotine blue leuco form + NADP+ two-electron transfer reaction ?
1.1.1.328 oxidized 2,6-dichlorophenol-indophenol + NADH + H+
-
 Paenarthrobacter nicotinovorans reduced 2,6-dichlorophenol-indophenol + NAD+
-
 r
1.1.1.328 oxidized 2,6-dichlorophenol-indophenol + NADPH + H+
-
 Paenarthrobacter nicotinovorans reduced 2,6-dichlorophenol-indophenol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.328 NAD(P)H-nicotine blue oxidoreductase
-
 Paenarthrobacter nicotinovorans
1.1.1.328 NAD(P)H-nicotine blue quinone oxidoreductase
-
 Paenarthrobacter nicotinovorans
1.1.1.328 nboR
-
 Paenarthrobacter nicotinovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.328 FMN
-
 Paenarthrobacter nicotinovorans
1.1.1.328 NADH NADH is prefered over NADPH Paenarthrobacter nicotinovorans
1.1.1.328 NADPH 70% of the rate with NADH Paenarthrobacter nicotinovorans

Expression

EC Number Organism Comment Expression
1.1.1.328 Paenarthrobacter nicotinovorans enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR additional information

General Information

EC Number General Information Comment Organism
1.1.1.328 physiological function enzyme is part of the nicotine catabolic pathway Paenarthrobacter nicotinovorans