Literature summary extracted from
Vidya, J.; Pandey, A.
Recombinant expression and characterization of L-asparaginase II from a moderately thermotolerant bacterial isolate (2012), Appl. Biochem. Biotechnol., 167, 973-980.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.5.1.1 |
gene ansB, cloning in Escherichia coli strain DH5alpha and expression as His6-tagged protein in Escherichia coli strain BL21(DE3) |
unidentified proteobacterium |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.5.1.1 |
0.89 |
- |
L-asparagine |
pH 7.0, 37°C, recombinant enzyme |
unidentified proteobacterium |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.5.1.1 |
36000 |
- |
x * 36000, recombinant His6-tagged enzyme, SDS-PAGE |
unidentified proteobacterium |
3.5.1.1 |
150000 |
- |
about, native PAGE |
unidentified proteobacterium |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.5.1.1 |
L-asparagine + H2O |
unidentified proteobacterium |
- |
L-aspartate + NH3 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.1.1 |
unidentified proteobacterium |
- |
Enterobacteriaceae, bacterium isolated from cow dung, gene ansB |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.5.1.1 |
recombinant soluble His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
unidentified proteobacterium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.1.1 |
L-asparagine + H2O |
- |
unidentified proteobacterium |
L-aspartate + NH3 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.5.1.1 |
oligomer |
x * 36000, recombinant His6-tagged enzyme, SDS-PAGE |
unidentified proteobacterium |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.5.1.1 |
L-asparaginase II |
- |
unidentified proteobacterium |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.5.1.1 |
30 |
37 |
- |
unidentified proteobacterium |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.5.1.1 |
30 |
70 |
activity range, sharp decrease in activity above 37°C |
unidentified proteobacterium |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.5.1.1 |
50 |
- |
the purified recombinant enzyme retains 38% of its initial activity after 10 min, 33% after 30 and 45 min, and 28% after 60 min of incubation |
unidentified proteobacterium |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.5.1.1 |
6 |
7 |
- |
unidentified proteobacterium |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.5.1.1 |
5.5 |
9 |
activity range, profile overview |
unidentified proteobacterium |