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Literature summary extracted from

  • Schmidberger, J.; James, A.; Edwards, R.; Naismith, J.; OHagan, D.
    Halomethane biosynthesis: Structure of a SAM-dependent halide methyltransferase from arabidopsis thaliana (2010), Angew. Chem. Int. Ed. Engl., 49, 3646-3648.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.9 His-tagged protein expressed in Escherichia coli Rosetta II (DE3) Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.9 co-crystallized with S-adenosyl-L-homocysteine Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.9 V23C not conserved active site residue, slightly improved activity, lowered stability Arabidopsis thaliana
2.1.1.9 Y172F reduced efficiency with Cl- Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.9 0.099
-
NCS- wild type protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.1.1.9 0.103
-
NCS- V23C mutant protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.1.1.9 0.141
-
NCS- Y172F mutant protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.9 Arabidopsis thaliana Q0WP12
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.9 immobilized metal ion affinity chromatography, His-tag remove Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.9 0.0434
-
substrate NCS-, V23C mutant protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.1.1.9 0.0436
-
substrate NCS-, wild type protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.1.1.9 0.046
-
substrate NCS-, Y172F mutant protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.9 thiocyanate + S-adenosyl-L-methionine
-
Arabidopsis thaliana methyl thiocyanate + S-adenosyl-L-homocysteine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.9 SAM-dependent halide methyltransferase
-
Arabidopsis thaliana