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Literature summary extracted from

  • Katayama, T.; Watanabe, M.; Tanaka, H.; Hino, M.; Miyakawa, T.; Ohki, T.; Ye, L.; Xie, C.; Yoshiyama, S.; Nakamura, A.; Ishikawa, R.; Tanokura, M.; Oiwa, K.; Kohama, K.
    Stimulatory effects of arachidonic acid on myosin ATPase activity and contraction of smooth muscle via myosin motor domain (2010), Am. J. Physiol. Heart Circ. Physiol., 298, H505-H514.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.1.8 arachidonic acid 0.0503 mM half-maximal effective concentration, 0.0263 mM half-maximal effective concentration in the presence of actin Gallus gallus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.8 His-tagged myosin head (Met1-Gln729) expressed in a baculovirus system Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.8 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.8 myosin with unphosphorylated regulatory light chain (20000 Da) from smooth muscle of gizzard; recombinant His-tagged myosin head: immobilized metal ion affinity chromatography (Ni2+) Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.8 gizzard smooth muscle
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.8 ATP + H2O + myosin bound to actin filament at position n
-
 Gallus gallus ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
5.6.1.8 CTP + H2O + myosin bound to actin filament at position n
-
 Gallus gallus CDP + phosphate + myosin bound to actin filament at position n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.8 myosin ATPase
-
 Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6.1.8 0.00892
-
 ATP unphosphorylated myosin, pH 7.5, 25°C Gallus gallus
5.6.1.8 0.0462
-
 ATP actin-activated, unphosphorylated myosin, pH 7.5, 25°C Gallus gallus
5.6.1.8 0.0678
-
 ATP phosphorylated myosin, pH 7.5, 25°C Gallus gallus
5.6.1.8 0.259
-
 ATP actin-activated, phosphorylated myosin, pH 7.5, 25°C Gallus gallus