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Literature summary extracted from

  • Benini, S.; Wilson, K.
    Structure of the Mycobacterium tuberculosis soluble inorganic pyrophosphatase Rv3628 at pH 7.0 (2011), Acta Crystallogr. Sect. F, 67, 866-870.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.1 purified recombinant Rv3628, hanging drop vapour diffusion method, mixing of 0.001 ml protein in150 mM Na HEPES, pH 7.0, and 0.001 ml reservoir solution, containing 1.6 M NaKHPO4, pH 7.0, and equilibration against 1 ml reservoir solution, X-ray diffraction structure determination and analysis at 1.5 A resolution, modeling Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 soluble
-
 Mycobacterium tuberculosis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 K+ determination of K+-binding sites Mycobacterium tuberculosis
3.6.1.1 Mg2+ required for catalysis Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Mycobacterium tuberculosis
-
 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Mycobacterium tuberculosis H37Rv
-
 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Mycobacterium tuberculosis P9WI55
-
-
3.6.1.1 Mycobacterium tuberculosis H37Rv P9WI55
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Mycobacterium tuberculosis 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 More structure comparison of family I enzymes, overview Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 inorganic pyrophosphatase
-
 Mycobacterium tuberculosis
3.6.1.1 Rv3628
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.6.1.1 evolution Mycobacterium tuberculosis and Mycobacterium leprae genomes include genes for the only two family I inorganic pyrophosphatases known to contain two histidines in the active site, structure comparison of family I enzymes, overview Mycobacterium tuberculosis
3.6.1.1 additional information His21 and His86 are not essential for diphosphate hydrolysis, but are responsible for a shift in the optimal pH for the reaction compared with the Escherichia coli enzyme Mycobacterium tuberculosis