Literature summary extracted from
Jeong, H.J.; Shin, J.S.; Ok, S.H.
Barley DNA-binding methionine aminopeptidase, which changes the localization from the nucleus to the cytoplasm by low temperature, is involved in freezing tolerance (2011), Plant Sci., 180, 53-60.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.11.18 |
glutathione-S-transferase fusion protein expressed in Escherichia coli BL21(DE3), expressed in Arabidopsis |
Hordeum vulgare |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.11.18 |
EDTA |
- |
Hordeum vulgare |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.4.11.18 |
nucleus |
translocated from the nucleus to the cytoplasm in response to low-temperature (observed in onion cells which transiently express a GFP fusion protein) |
Hordeum vulgare |
5634 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.11.18 |
Co2+ |
- |
Hordeum vulgare |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.11.18 |
Hordeum vulgare |
E0A9F0 |
cv. Dongbori |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.11.18 |
commercial GST gene fusion purification system, GST protein removed |
Hordeum vulgare |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.11.18 |
Met-S-Gly-Phe + H2O |
- |
Hordeum vulgare |
L-methionine + ? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.11.18 |
methionine aminopeptidase |
nuclear localization signal at C-terminal end |
Hordeum vulgare |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
3.4.11.18 |
Hordeum vulgare |
low temperature, abscisic acid treatment |
up |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.4.11.18 |
physiological function |
stronger freezing tolerance (transgenic Arabidopsis plants) |
Hordeum vulgare |