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Literature summary extracted from

  • Nieuwenhuizen, N.J.; Maddumage, R.; Tsang, G.K.; Fraser, L.G.; Cooney, J.M.; De Silva, H.N.; Green, S.; Richardson, K.A.; Atkinson, R.G.
    Mapping, complementation, and targets of the cysteine protease actinidin in kiwifruit (2012), Plant Physiol., 158, 376-388.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.14 expressed in Nicotiana benthamiana T1, T2, T3, and T4 transgenic lines Actinidia chinensis
3.4.22.14 expressed in Nicotiana benthamiana T1, T2, T3, and T4 transgenic lines Actinidia deliciosa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.14 P75Q the mutant shows reduced activity compared tot he wild type enzyme Actinidia chinensis
3.4.22.14 P75Q the mutant shows reduced activity compared tot he wild type enzyme Actinidia deliciosa
3.4.22.14 S68G inactive Actinidia chinensis
3.4.22.14 S68G inactive Actinidia deliciosa
3.4.22.14 S68G/P75Q the mutant shows reduced activity compared tot he wild type enzyme Actinidia chinensis
3.4.22.14 S68G/P75Q the mutant shows reduced activity compared tot he wild type enzyme Actinidia deliciosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.14 24000
-
x * 24000, SDS-PAGE Actinidia chinensis
3.4.22.14 24000
-
x * 24000, SDS-PAGE Actinidia deliciosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.14 Actinidia chinensis
-
variety YellowA
-
3.4.22.14 Actinidia deliciosa
-
variety Hayward
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.14 fruit
-
Actinidia chinensis
-
3.4.22.14 fruit
-
Actinidia deliciosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.14 Gelatin + H2O
-
Actinidia chinensis ?
-
?
3.4.22.14 Gelatin + H2O
-
Actinidia deliciosa ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.22.14 ? x * 24000, SDS-PAGE Actinidia chinensis
3.4.22.14 ? x * 24000, SDS-PAGE Actinidia deliciosa

Synonyms

EC Number Synonyms Comment Organism
3.4.22.14 ACT1A-1 major isoform Actinidia chinensis
3.4.22.14 ACT1A-1 major isoform Actinidia deliciosa
3.4.22.14 ACT1A-2 minor isoform Actinidia chinensis
3.4.22.14 ACT1A-2 minor isoform Actinidia deliciosa
3.4.22.14 actinidin
-
Actinidia chinensis
3.4.22.14 actinidin
-
Actinidia deliciosa