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Literature summary extracted from

  • Funk, C.; Croteau, R.
    Induction and characterization of a cytochrome P-450-dependent camphor hydroxylase in tissue cultures of common sage (Salvia officinalis) (1993), Plant Physiol., 101, 1231-1237.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.161 FAD inclusion of both FAD and FMN in the incubation mixture enhances hydroxylation activity 2.4fold Salvia officinalis
1.14.13.161 FMN inclusion of both FAD and FMN in the incubation mixture enhances hydroxylation activity 2.4fold Salvia officinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.161 clotrimazole 84% inhibition at 0.05 mM Salvia officinalis
1.14.13.161 CO 73% inhibition at CO:O2 ratio of 9:1, activity is partially restored by blue light Salvia officinalis
1.14.13.161 cytochrome c 95% inhibition at 0.05 mM Salvia officinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.161 0.034
-
 (+)-Camphor pH 7.0, 32°C Salvia officinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.161 membrane microsomal membrane Salvia officinalis 16020
-
1.14.13.161 microsome microsomal membrane Salvia officinalis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.161 MnCl2 upon treatment of sage suspension cultures with 30 mM MnCI2, camphor-6-hydroxylase activity is induced up to 7fold Salvia officinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.161 58000
-
 x * 58000, SDS-PAGE Salvia officinalis

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.161 Salvia officinalis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.161 cell suspension culture
-
 Salvia officinalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.161 (+)-camphor + NADPH + H+ + O2
-
 Salvia officinalis (+)-6-exo-hydroxycamphor + NADP+ + H2O
-
 ?
1.14.13.161 additional information the microsomal camphor-6-exo-hydroxylase system requires molecular oxygen and a reduced pyridine nucleotide Salvia officinalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.161 ? x * 58000, SDS-PAGE Salvia officinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.161 7
-
-
 Salvia officinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.161 6.5 7.5 more than 60% of maximum activity within Salvia officinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.161 NADH 21% of the rate with NADPH Salvia officinalis
1.14.13.161 NADPH
-
 Salvia officinalis