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Literature summary extracted from

  • Pak, F.E.; Gropper, S.; Dai, W.D.; Havkin-Frenkel, D.; Belanger, F.C.
    Characterization of a multifunctional methyltransferase from the orchid Vanilla planifolia (2004), Plant Cell Rep., 22, 959-966.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 cloning from phage library, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in strain JM109 Vanilla planifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.021
-
 5-hydroxyferulic acid ethyl ester pH and temperature not specified in the publication Vanilla planifolia
2.1.1.68 0.023
-
 caffeic acid ethyl ester pH and temperature not specified in the publication Vanilla planifolia
2.1.1.68 0.032
-
 caffeoyl aldehyde pH and temperature not specified in the publication Vanilla planifolia
2.1.1.68 0.041
-
 5-hydroxyconiferaldehyde pH and temperature not specified in the publication Vanilla planifolia
2.1.1.68 0.091
-
 5-hydroxyferulic acid pH and temperature not specified in the publication Vanilla planifolia
2.1.1.68 0.25
-
 caffeic acid pH and temperature not specified in the publication Vanilla planifolia
2.1.1.68 0.255
-
 3,4-Dihydroxybenzaldehyde pH and temperature not specified in the publication Vanilla planifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.68 40659
-
 x * 42000, SDS-PAGE, x * 40659, sequence calculation Vanilla planifolia
2.1.1.68 42000
-
 x * 42000, SDS-PAGE, x * 40659, sequence calculation Vanilla planifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde Vanilla planifolia 3,4-dihydroxybenzaldehyde is not the preferred substrate S-adenosyl-L-homocysteine + vanillin i.e. 4-hydroxy-3-methoxybenzaldehyde ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Vanilla planifolia Q6Q796
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.68 native enzyme partially by adenosine-agarose affinity chromatography, 18.2fold for 3,4-dihydroxybenzaldehyde-O-methyltransferase activity and 16.7fold for caffeic acid O-methyltransferase activity Vanilla planifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.68 leaf low expression level Vanilla planifolia
-
2.1.1.68 additional information tissue distribution by immunohistochemic analysis Vanilla planifolia
-
2.1.1.68 pod low expression level Vanilla planifolia
-
2.1.1.68 root strong expression level Vanilla planifolia
-
2.1.1.68 stem strong expression level Vanilla planifolia
-
2.1.1.68 tissue culture strong expression level Vanilla planifolia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 additional information substrate specificity, overview Vanilla planifolia ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde 3,4-dihydroxybenzaldehyde is not the preferred substrate Vanilla planifolia S-adenosyl-L-homocysteine + vanillin i.e. 4-hydroxy-3-methoxybenzaldehyde ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde 3,4-dihydroxy-benzaldehyde is not the preferred substrate Vanilla planifolia S-adenosyl-L-homocysteine + vanillin i.e. 4-hydroxy-3-methoxybenzaldehyde ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
-
 Vanilla planifolia S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Vanilla planifolia S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid ethyl ester
-
 Vanilla planifolia S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeic acid
-
 Vanilla planifolia S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeic acid ethyl ester
-
 Vanilla planifolia S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl aldehyde
-
 Vanilla planifolia S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.68 ? x * 42000, SDS-PAGE, x * 40659, sequence calculation Vanilla planifolia

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 3,4-dihydroxybenzaldehyde-O-methyltransferase
-
 Vanilla planifolia
2.1.1.68 caffeic acid O-methyltransferase
-
 Vanilla planifolia
2.1.1.68 COMT
-
 Vanilla planifolia
2.1.1.68 DOMT
-
 Vanilla planifolia
2.1.1.68 O-methytransferase
-
 Vanilla planifolia
2.1.1.68 OMT
-
 Vanilla planifolia

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.68 S-adenosyl-L-methionine
-
 Vanilla planifolia

General Information

EC Number General Information Comment Organism
2.1.1.68 metabolism 3,4-dihydroxybenzaldehyde is not the preferred substrate. The higher catalytic efficiency of the purified recombinant enzyme with the substrates caffeoyl aldehyde and 5-hydroxyconiferaldehyde, and its tissue distribution, suggest this methyltransferase may primarily unction in lignin biosynthesis Vanilla planifolia