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Literature summary extracted from

  • Han, Y.P.; Li, Z.Y.; Li, B.C.; Sun, X.; Zhu, C.C.; Ling, X.T.; Zheng, H.Q.; Wu, Z.D.; Lv, Z.Y.
    Molecular cloning and characterization of a cathepsin B from Angiostrongylus cantonensis (2011), Parasitol. Res., 109, 369-378.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 L-cysteine cathepsin B is activated at 22°C for 5 min in 100 mM sodium acetate, 1 mM DTT, 2 mM EDTA, 4 mM cysteine, pH 5.5 Angiostrongylus cantonensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.1 expressed in Escherichia coli BL21(DE3) cells Angiostrongylus cantonensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 cystatin the enzyme retains 10% and 5% of its initial activity, after 30 min of incubation at 37°C, in the presence of 75 and 100 nM recombinant cystatin, respectively Angiostrongylus cantonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 40000
-
 x * 40000, SDS-PAGE Angiostrongylus cantonensis
3.4.22.1 40850
-
 x * 40850, calculated from amino acid sequence Angiostrongylus cantonensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 ovalbumin + H2O Angiostrongylus cantonensis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Angiostrongylus cantonensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1 Talon affinity column chromatography Angiostrongylus cantonensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 adult
-
 Angiostrongylus cantonensis
-
3.4.22.1 larva the enzyme is expressed significantly higher in the fourth-stage larvae and the fifth-stage larvae than that in the third-stage larvae and adult worms Angiostrongylus cantonensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Angiostrongylus cantonensis N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 ovalbumin + H2O
-
 Angiostrongylus cantonensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.1 ? x * 40000, SDS-PAGE Angiostrongylus cantonensis
3.4.22.1 ? x * 40850, calculated from amino acid sequence Angiostrongylus cantonensis

Synonyms

EC Number Synonyms Comment Organism
3.4.22.1 CPB
-
 Angiostrongylus cantonensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.1 37
-
-
 Angiostrongylus cantonensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.1 17 57 the activity is relatively stable at 17°C and 27°C, retaining about 95% and 98% activity, respectively. The relative activities at 47°C and 57°C are 31% and 13% of that at 37°C, respectively Angiostrongylus cantonensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.1 99
-
 the activity drops dramatically within the first 30 min and 70% activity is lost after 30 min of incubation at 99°C Angiostrongylus cantonensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 5
-
-
 Angiostrongylus cantonensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.1 4 6 there is 60% activity at pH 4.0. The activity decreases significantly below pH 4.0 or above pH 5.0, and 5% activity is detected at pH 6.0 Angiostrongylus cantonensis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.22.1 0.06
-
 at pH 5.0 and 37°C Angiostrongylus cantonensis cystatin