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Literature summary extracted from

  • Van Der Geize, R.; Hessels, G.; Van Gerwen, R.; Van Der Meijden, P.; Dijkhuizen, L.
    Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9alpha-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis strain SQ1 (2002), Mol. Microbiol., 45, 1007-1018.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.30 additional information unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG2 (kshA mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected Rhodococcus erythropolis
1.14.15.30 additional information unmarked in frame gene deletion mutant of parent strain Rhodococcus erythropolis strain SQ1, designated strain RG4 (kshB mutant), is unable to grow on steroid substrates androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione, whereas growth on 9alpha-hydroxy-4-androstene-3,17-dione is not affected. Strain RG4 is also impaired in sterol degradation Rhodococcus erythropolis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.30 Fe2+ contains non-heme iron Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.30 Rhodococcus erythropolis Q8KQH9 component KshB of 3-ketosteroid 9alpha-hydroxylase
-
1.14.15.30 Rhodococcus erythropolis Q8KQI0 component KshA of 3-ketosteroid 9alpha-hydroxylase
-
1.14.15.30 Rhodococcus erythropolis SQ1 Q8KQH9 component KshB of 3-ketosteroid 9alpha-hydroxylase
-
1.14.15.30 Rhodococcus erythropolis SQ1 Q8KQI0 component KshA of 3-ketosteroid 9alpha-hydroxylase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.30 4-androstene-3,17-dione + O2 + NADH + H+
-
 Rhodococcus erythropolis 9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
 ?
1.14.15.30 4-androstene-3,17-dione + O2 + NADH + H+
-
 Rhodococcus erythropolis SQ1 9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus erythropolis 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.30 androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Rhodococcus erythropolis SQ1 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.30 3-ketosteroid 9alpha-hydroxylase
-
 Rhodococcus erythropolis
1.14.15.30 KSH
-
 Rhodococcus erythropolis
1.14.15.30 KshA terminal oxygenase component of 3-ketosteroid 9alpha-hydroxylase, kshA encodes both androsta-1,4-diene-3,17-dione and 4-androstene-3,17-dione 9alpha-hydroxylase activity Rhodococcus erythropolis
1.14.15.30 KshB the KshB protein can be assigned to the class IA oxygenase ferredoxin reductases and represents the ferredoxin reductase component of 3-ketosteroid 9alpha-hydroxylase Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.30 [2Fe-2S]-center
-
 Rhodococcus erythropolis