BRENDA - Enzyme Database

Enzymic and genetic basis for bacterial growth on malonate

Dimroth, P.; Hilbi, H.; Mol. Microbiol. 25, 3-10 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.187
gene mdcA, encoded in the malonate decarboxylase operon mdc, genetic organization
Klebsiella pneumoniae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.187
60800
-
x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
Klebsiella pneumoniae
2.3.1.187
61200
-
x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
Malonomonas rubra
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.187
malonate + acetyl-[acyl-carrier protein]
Klebsiella pneumoniae
-
acetate + malonyl-[acyl-carrier protein]
-
-
?
2.3.1.187
malonate + acetyl-[acyl-carrier protein]
Malonomonas rubra
-
acetate + malonyl-[acyl-carrier protein]
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.187
Klebsiella pneumoniae
-
gene mdcA, encoded in the malonate decarboxylase operon mdc
-
2.3.1.187
Malonomonas rubra
-
gene madA, encoded in the malonate decarboxylase operon mad
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.187
isolated soluble native transferase component of the malonate decarboxylase
Malonomonas rubra
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.187
malonate + acetyl-[acyl-carrier protein]
-
718124
Klebsiella pneumoniae
acetate + malonyl-[acyl-carrier protein]
-
-
-
?
2.3.1.187
malonate + acetyl-[acyl-carrier protein]
-
718124
Malonomonas rubra
acetate + malonyl-[acyl-carrier protein]
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.187
?
x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
Klebsiella pneumoniae
2.3.1.187
?
x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
Malonomonas rubra
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.187
gene mdcA, encoded in the malonate decarboxylase operon mdc, genetic organization
Klebsiella pneumoniae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.187
60800
-
x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
Klebsiella pneumoniae
2.3.1.187
61200
-
x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
Malonomonas rubra
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.187
malonate + acetyl-[acyl-carrier protein]
Klebsiella pneumoniae
-
acetate + malonyl-[acyl-carrier protein]
-
-
?
2.3.1.187
malonate + acetyl-[acyl-carrier protein]
Malonomonas rubra
-
acetate + malonyl-[acyl-carrier protein]
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.187
isolated soluble native transferase component of the malonate decarboxylase
Malonomonas rubra
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.187
malonate + acetyl-[acyl-carrier protein]
-
718124
Klebsiella pneumoniae
acetate + malonyl-[acyl-carrier protein]
-
-
-
?
2.3.1.187
malonate + acetyl-[acyl-carrier protein]
-
718124
Malonomonas rubra
acetate + malonyl-[acyl-carrier protein]
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.187
?
x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
Klebsiella pneumoniae
2.3.1.187
?
x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
Malonomonas rubra
General Information
EC Number
General Information
Commentary
Organism
2.3.1.187
metabolism
to achieve decarboxylation of malonate, the key reaction in the decomposition of this compound, the substrate must be activated by the malonate decarboxylase to a malonyl thio-enzyme ester involving an activation module that comprises the acetylation of a specific thiol group of an acyl carrier protein, ACP, and the transfer of the ACP moiety to malonate, yielding malonyl-S-ACP and acetate. The malonyl-S-ACP is subsequently decarboxylated with regeneration of the acetyl-ACP. Malonate decarboxylation pathway, overview
Klebsiella pneumoniae
2.3.1.187
metabolism
to achieve decarboxylation of malonate, the key reaction in the decomposition of this compound, the substrate must be activated by the malonate decarboxylase to a malonyl thio-enzyme ester involving an activation module that comprises the acetylation of a specific thiol group of an acyl carrier protein, ACP, and the transfer of the ACP moiety to malonate, yielding malonyl-S-ACP and acetate. The malonyl-S-ACP is subsequently decarboxylated with regeneration of the acetyl-ACP. Malonate decarboxylation pathway, overview
Malonomonas rubra
2.3.1.187
additional information
the acetyl-S-ACP:malonate ACP transferase is a component of the malonate decarboxylase complex
Klebsiella pneumoniae
2.3.1.187
additional information
the acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase is a component of the malonate decarboxylase complex
Malonomonas rubra
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.3.1.187
metabolism
to achieve decarboxylation of malonate, the key reaction in the decomposition of this compound, the substrate must be activated by the malonate decarboxylase to a malonyl thio-enzyme ester involving an activation module that comprises the acetylation of a specific thiol group of an acyl carrier protein, ACP, and the transfer of the ACP moiety to malonate, yielding malonyl-S-ACP and acetate. The malonyl-S-ACP is subsequently decarboxylated with regeneration of the acetyl-ACP. Malonate decarboxylation pathway, overview
Klebsiella pneumoniae
2.3.1.187
metabolism
to achieve decarboxylation of malonate, the key reaction in the decomposition of this compound, the substrate must be activated by the malonate decarboxylase to a malonyl thio-enzyme ester involving an activation module that comprises the acetylation of a specific thiol group of an acyl carrier protein, ACP, and the transfer of the ACP moiety to malonate, yielding malonyl-S-ACP and acetate. The malonyl-S-ACP is subsequently decarboxylated with regeneration of the acetyl-ACP. Malonate decarboxylation pathway, overview
Malonomonas rubra
2.3.1.187
additional information
the acetyl-S-ACP:malonate ACP transferase is a component of the malonate decarboxylase complex
Klebsiella pneumoniae
2.3.1.187
additional information
the acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase is a component of the malonate decarboxylase complex
Malonomonas rubra