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Literature summary extracted from

  • Carvalho, A.F.; Boscolo, M.; da Silva, R.; Ferreira, H.; Gomes, E.
    Purification and characterization of the alpha-glucosidase produced by thermophilic fungus Thermoascus aurantiacus CBMAI 756 (2010), J. Microbiol., 48, 452-459.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Ag+
-
Thermoascus aurantiacus
3.2.1.20 Cr3+
-
Thermoascus aurantiacus
3.2.1.20 Hg2+
-
Thermoascus aurantiacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 additional information the metal ions Na+, Ba2+, Co2+, Ni2+, Mg2+, Mn2+, Al3+, Zn2+, Ca2+ do not or poorly influence the enzyme activity Thermoascus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 83000
-
x * 83000, SDS-PAGE Thermoascus aurantiacus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Thermoascus aurantiacus
-
-
-
3.2.1.20 Thermoascus aurantiacus CBMAI 756
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 native enzyme 172fold by ultrafiltration, ammonium sulfate fractionation, anion exchange chromatograpyh, and two different steps of gel filtration Thermoascus aurantiacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 404
-
purified enzyme, pH 4.5, 70°C Thermoascus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 maltoheptaose + H2O
-
Thermoascus aurantiacus ?
-
?
3.2.1.20 maltoheptaose + H2O
-
Thermoascus aurantiacus CBMAI 756 ?
-
?
3.2.1.20 maltohexaose + H2O
-
Thermoascus aurantiacus ?
-
?
3.2.1.20 maltohexaose + H2O
-
Thermoascus aurantiacus CBMAI 756 ?
-
?
3.2.1.20 maltopentaose + H2O
-
Thermoascus aurantiacus ?
-
?
3.2.1.20 maltopentaose + H2O
-
Thermoascus aurantiacus CBMAI 756 ?
-
?
3.2.1.20 maltose + H2O
-
Thermoascus aurantiacus alpha-D-glucose + D-glucose
-
?
3.2.1.20 maltotetraose + H2O
-
Thermoascus aurantiacus ?
-
?
3.2.1.20 maltotetraose + H2O
-
Thermoascus aurantiacus CBMAI 756 ?
-
?
3.2.1.20 maltotriose + H2O
-
Thermoascus aurantiacus ?
-
?
3.2.1.20 additional information the enzyme shows a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides, the enzymatic activity decreases following an increase in the polymerization degree of short maltooligosaccharides, low activity with maltoheptaose, no activity with starch Thermoascus aurantiacus ?
-
?
3.2.1.20 additional information the enzyme shows a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides, the enzymatic activity decreases following an increase in the polymerization degree of short maltooligosaccharides, low activity with maltoheptaose, no activity with starch Thermoascus aurantiacus CBMAI 756 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 83000, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 70
-
-
Thermoascus aurantiacus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 50 80 activity range, profile, overview Thermoascus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 40 60 purified enzyme, 60% activity remaining Thermoascus aurantiacus
3.2.1.20 70
-
purified enzyme, 30% activity remaining Thermoascus aurantiacus
3.2.1.20 80
-
purified enzyme, no activity remaining Thermoascus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.5
-
-
Thermoascus aurantiacus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 2.5 7 activity range, profile, overview Thermoascus aurantiacus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 3 9 purified enzyme, completely stable Thermoascus aurantiacus