EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | Ag+ | - |
Thermoascus aurantiacus | |
3.2.1.20 | Cr3+ | - |
Thermoascus aurantiacus | |
3.2.1.20 | Hg2+ | - |
Thermoascus aurantiacus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | additional information | the metal ions Na+, Ba2+, Co2+, Ni2+, Mg2+, Mn2+, Al3+, Zn2+, Ca2+ do not or poorly influence the enzyme activity | Thermoascus aurantiacus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 83000 | - |
x * 83000, SDS-PAGE | Thermoascus aurantiacus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Thermoascus aurantiacus | - |
- |
- |
3.2.1.20 | Thermoascus aurantiacus CBMAI 756 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.20 | native enzyme 172fold by ultrafiltration, ammonium sulfate fractionation, anion exchange chromatograpyh, and two different steps of gel filtration | Thermoascus aurantiacus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 404 | - |
purified enzyme, pH 4.5, 70°C | Thermoascus aurantiacus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | maltoheptaose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
3.2.1.20 | maltoheptaose + H2O | - |
Thermoascus aurantiacus CBMAI 756 | ? | - |
? | |
3.2.1.20 | maltohexaose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
3.2.1.20 | maltohexaose + H2O | - |
Thermoascus aurantiacus CBMAI 756 | ? | - |
? | |
3.2.1.20 | maltopentaose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
3.2.1.20 | maltopentaose + H2O | - |
Thermoascus aurantiacus CBMAI 756 | ? | - |
? | |
3.2.1.20 | maltose + H2O | - |
Thermoascus aurantiacus | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | maltotetraose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
3.2.1.20 | maltotetraose + H2O | - |
Thermoascus aurantiacus CBMAI 756 | ? | - |
? | |
3.2.1.20 | maltotriose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
3.2.1.20 | additional information | the enzyme shows a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides, the enzymatic activity decreases following an increase in the polymerization degree of short maltooligosaccharides, low activity with maltoheptaose, no activity with starch | Thermoascus aurantiacus | ? | - |
? | |
3.2.1.20 | additional information | the enzyme shows a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides, the enzymatic activity decreases following an increase in the polymerization degree of short maltooligosaccharides, low activity with maltoheptaose, no activity with starch | Thermoascus aurantiacus CBMAI 756 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.20 | ? | x * 83000, SDS-PAGE | Thermoascus aurantiacus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 70 | - |
- |
Thermoascus aurantiacus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 50 | 80 | activity range, profile, overview | Thermoascus aurantiacus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 40 | 60 | purified enzyme, 60% activity remaining | Thermoascus aurantiacus |
3.2.1.20 | 70 | - |
purified enzyme, 30% activity remaining | Thermoascus aurantiacus |
3.2.1.20 | 80 | - |
purified enzyme, no activity remaining | Thermoascus aurantiacus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 4.5 | - |
- |
Thermoascus aurantiacus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 2.5 | 7 | activity range, profile, overview | Thermoascus aurantiacus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 3 | 9 | purified enzyme, completely stable | Thermoascus aurantiacus |