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Literature summary extracted from

  • Shapiro, A.B.; Gao, N.; Thresher, J.; Walkup, G.K.; Whiteaker, J.
    A high-throughput absorbance-based assay for methionine produced by methionine aminopeptidase using S-adenosyl-L-methionine synthetase (2011), J. Biomol. Screen., 16, 494-505.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.18 expressed in Escherichia coli BL21(DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.18 5-(2-chlorophenyl)-2-furohydrazide
-
 Escherichia coli
3.4.11.18 EDTA
-
 Escherichia coli
3.4.11.18 EGTA
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.18 1.2
-
 Met-Gly-Met-Met Mn2+-activated enzyme, pH 7.5, 21°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.18 Mn2+ activation constant 0.003 mM Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.18 Escherichia coli
-
-
-
3.4.11.18 Escherichia coli K-12 MG1655
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.18 ion exchange chromatography (Q-Sepharose), hydrophobic interaction chromatography (Phenyl Sepharose), gel filtration (Sephacryl S-200) Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.18 Met-Gly-Met-Met + H2O
-
 Escherichia coli L-methionine + Gly-Met-Met
-
 ?
3.4.11.18 Met-Gly-Met-Met + H2O
-
 Escherichia coli K-12 MG1655 L-methionine + Gly-Met-Met
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.18 methionine aminopeptidase
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.18 0.002
-
 Mn2+-activated enzyme (0.004 mM MnCl2), pH 7.5, 21°C Escherichia coli 5-(2-chlorophenyl)-2-furohydrazide