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Literature summary extracted from

  • Wang, Q.; Hillwig, M.; Okada, K.; Yamazaki, K.; Wu, Y.; Swaminathan, S.; Yamane, H.; Peters, R.
    Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster (2012), J. Biol. Chem., 287, 6159-6168.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.123 expressed in Escherichia coli C41 cells Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.123 0.002
-
syn-pimaradiene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.004
-
ent-cassadiene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.004
-
ent-pimaradiene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.005
-
ent-kaurene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.007
-
ent-sandaracopimaradien-3beta-ol pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.015
-
ent-isokaurene pH and temperature not specified in the publication Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.123 ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa
-
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.112 Oryza sativa
-
-
-
1.14.14.123 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.123 ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa ?
-
?
1.14.14.123 ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa ?
-
?
1.14.14.123 ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa ?
-
?
1.14.14.123 ent-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa ?
-
?
1.14.14.123 ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.123 additional information no reaction with syn-pimaradien-19-oic acid, syn-stemodene, syn-stemarene, or syn-labdatriene Oryza sativa ?
-
?
1.14.14.123 syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa momilactone A + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.112 CYP76M7
-
Oryza sativa
1.14.14.123 CYP76M8
-
Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.123 0.006
-
ent-cassadiene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.006
-
syn-pimaradiene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.009
-
ent-kaurene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.009
-
ent-pimaradiene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.011
-
ent-sandaracopimaradien-3beta-ol pH and temperature not specified in the publication Oryza sativa
1.14.14.123 0.015
-
ent-isokaurene pH and temperature not specified in the publication Oryza sativa

General Information

EC Number General Information Comment Organism
1.14.14.112 malfunction RNAi-mediated double knockdown of CYP76M7 and -8 suppresses elicitor inducible phytocassane production, indicating a role for these monooxygenases in phytocassane biosynthesis Oryza sativa
1.14.14.112 physiological function the enzyme mediates an early step in biosynthesis of the derived phytocassane class of phytoalexins Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.123 1
-
ent-isokaurene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 1.5
-
ent-cassadiene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 1.6
-
ent-sandaracopimaradien-3beta-ol pH and temperature not specified in the publication Oryza sativa
1.14.14.123 1.8
-
ent-kaurene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 2.3
-
ent-pimaradiene pH and temperature not specified in the publication Oryza sativa
1.14.14.123 2.6
-
syn-pimaradiene pH and temperature not specified in the publication Oryza sativa