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Literature summary extracted from

  • Zhang, L.; Conway, J.F.; Thibodeau, P.H.
    Calcium-induced folding and stabilization of the Pseudomonas aeruginosa alkaline protease (2012), J. Biol. Chem., 287, 4311-4322.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.40 expression of wild-type and mutant AP proteins and domains at high levels in inclusion bodies Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.40 additional information generation of a series of full-length and truncation mutants for structural and functional studies, overview. DELTAN-AP shows increased thermal sensitivity compared with the wild-type enzyme. At 42°C, protease activity decreases by 50%, and at 55°C, protease activity decreases by 90% when compared with the full-length AP in 2 mM Ca2+ Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Ca2+ calcium-regulated alkaline protease, role of Ca2+ in enzyme folding and activation: Ca2+binding induces RTX folding, which serves to chaperone the folding of the protease domain. Subsequent association of the RTX domain with an N-terminal alpha-helix stabilizes AP, Ca2+ regulates the RTX family of virulence factors, overview Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40 recombinant refolded His-tagged wild-type and mutant AP proteins and domains from inclusion bodies by nickel affinity chromatography Pseudomonas aeruginosa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.24.40 recombinant His-tagged wild-type and mutant AP proteins and domains under denaturing conditions are refolded from inclusion bodies using either urea for RTX, or guanidine HCl for full-length and truncation mutants Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.40 calcium-regulated alkaline protease
-
 Pseudomonas aeruginosa
3.4.24.40 Pseudomonas aeruginosa alkaline protease
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.40 additional information
-
 comparison of full-length and truncation mutants, DELTAN-AP shows increased thermal sensitivity compared with the wild-type enzyme. At 42°C, protease activity decreases by 50%, and at 55°C, protease activity decreases by 90% when compared with the full-length AP in 2 mM Ca2+ Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
3.4.24.40 evolution the calcium-regulated alkaline protease is a member of the repeats in toxin, RTX, family of proteins Pseudomonas aeruginosa
3.4.24.40 physiological function the calcium-regulated alkaline protease is implicated in multiple modes of infection Pseudomonas aeruginosa