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Literature summary extracted from

  • Ferguson, T.; Soares, J.; Lienard, T.; Gottschalk, G.; Krzycki, J.
    RamA, a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea (2009), J. Biol. Chem., 284, 2285-2295.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.248 RamA a 60-kDa monomeric iron sulfur protein, is a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea, it is required for in vitro ATP-dependent reductive activation of methylamine:CoM methyl transfer mediating the ATP-dependent reductive activation of Co(II) corrinoid to the Co(I) state for the monomethylamine corrinoid protein, MtmC, overview. RamA affects both lag times and rates during assay of MMA:CoM methyl transfer Methanosarcina barkeri
2.1.1.249 RamA a 60-kDa monomeric iron sulfur protein, is a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea, it is required for in vitro ATP-dependent reductive activation of dimethylamine:CoM methyl transfer mediating the ATP-dependent reductive activation of Co(II) corrinoid to the Co(I) state for the trimethylamine corrinoid protein, MtbC, overview Methanosarcina barkeri
2.1.1.250 RamA a 60-kDa monomeric iron sulfur protein, is a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea, it is required for in vitro ATP-dependent reductive activation of trimethylamine:CoM methyl transfer mediating the ATP-dependent reductive activation of Co(II) corrinoid to the Co(I) state for the trimethylamine corrinoid protein, MttC, overview Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri catalytic cycle of MMA:CoM methyl transfer, overview a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.248 Methanosarcina barkeri
-
-
-
2.1.1.249 Methanosarcina barkeri
-
 gene mtbB
-
2.1.1.250 Methanosarcina barkeri O93658 gene mttB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.248 native MtmB from monomethylamine-grown cells, by two different steps of anionexchange chromatography, followed by gel filtration and another step of anion exchange chromatography to near homogeneity, co-purification with the monomethylamine-specific corrinoid protein MtmC Methanosarcina barkeri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.248 culture condition:monomethylamine-grown cell
-
 Methanosarcina barkeri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
2.1.1.248 methylamine + a [Co(I) methylamine-specific corrinoid protein] catalytic cycle of MMA:CoM methyl transfer, overview Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.248 corrinoid-dependent methylamine methyltransferase
-
 Methanosarcina barkeri
2.1.1.248 MMA:CoM methyltransferase
-
 Methanosarcina barkeri
2.1.1.248 MtmB
-
 Methanosarcina barkeri
2.1.1.249 DMA:CoM methyltransferase
-
 Methanosarcina barkeri
2.1.1.249 MtcB
-
 Methanosarcina barkeri
2.1.1.250 MttB
-
 Methanosarcina barkeri
2.1.1.250 TMA:CoM methyltransferase
-
 Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.248 metabolism archaeal methane formation from methylamines is initiated by distinct methyltransferases with specificity for monomethylamine, dimethylamine, or trimethylamine. Each methylamine methyltransferase methylates a cognate corrinoid protein, which is subsequently demethylated by a second methyltransferase to form methyl-coenzyme M, the direct methane precursor Methanosarcina barkeri
2.1.1.249 metabolism archaeal methane formation from methylamines is initiated by distinct methyltransferases with specificity for monomethylamine, dimethylamine, or trimethylamine. Each methylamine methyltransferase methylates a cognate corrinoid protein, which is subsequently demethylated by a second methyltransferase to form methyl-coenzyme M, the direct methane precursor Methanosarcina barkeri
2.1.1.250 metabolism archaeal methane formation from methylamines is initiated by distinct methyltransferases with specificity for monomethylamine, dimethylamine, or trimethylamine. Each methylamine methyltransferase methylates a cognate corrinoid protein, which is subsequently demethylated by a second methyltransferase to form methyl-coenzyme M, the direct methane precursor Methanosarcina barkeri