BRENDA - Enzyme Database

Purification and characterization of Chinese hamster phosphatidylserine synthase 2

Kuge, O.; Hasegawa, K.; Ohsawa, T.; Saito, K.; Nishijima, M.; J. Biol. Chem. 278, 42692-42698 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.8.29
expression of FLAG- and HA peptide-tagged form
Cricetulus griseus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.29
additional information
not inhibitory: phosphatidylethanolamine, phosphatidylcholine
Cricetulus griseus
2.7.8.29
phosphatidylserine
inhibition of purified enzyme and the crude enzyme in a membrane fraction
Cricetulus griseus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.29
55000
-
x * 55000, SDS-PAGE
Cricetulus griseus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.29
Cricetulus griseus
O08888
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.29
recombinant protein
Cricetulus griseus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.8.29
0.26
-
substrate L-serine, pH 7.5, 37░C
Cricetulus griseus
2.7.8.29
0.51
-
substrate L-1-phosphatidylethanolamine, pH 7.5, 37░C
Cricetulus griseus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
-
717768
Cricetulus griseus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.29
?
x * 55000, SDS-PAGE
Cricetulus griseus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.29
expression of FLAG- and HA peptide-tagged form
Cricetulus griseus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.29
additional information
not inhibitory: phosphatidylethanolamine, phosphatidylcholine
Cricetulus griseus
2.7.8.29
phosphatidylserine
inhibition of purified enzyme and the crude enzyme in a membrane fraction
Cricetulus griseus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.29
55000
-
x * 55000, SDS-PAGE
Cricetulus griseus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.29
recombinant protein
Cricetulus griseus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.8.29
0.26
-
substrate L-serine, pH 7.5, 37░C
Cricetulus griseus
2.7.8.29
0.51
-
substrate L-1-phosphatidylethanolamine, pH 7.5, 37░C
Cricetulus griseus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
-
717768
Cricetulus griseus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.29
?
x * 55000, SDS-PAGE
Cricetulus griseus