EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.99.46 | gene rutA, recombinant overexpression of His-tagged RutA in ASKA strain JW0997 | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.110 | additional information | construction of diverse rutB-deficient strains, overview | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.46 | thymine + FMNH2 + O2 | Escherichia coli | - |
(Z)-2-methylureidoacrylate peracid + FMN | - |
? | |
1.14.99.46 | uracil + FMNH2 + O2 | Escherichia coli | - |
(Z)-3-ureidoacrylate peracid + FMN | - |
? | |
3.5.1.110 | ureidoacrylate peracid + H2O | Escherichia coli | - |
peroxyaminoacrylate + NH3 + CO2 | - |
? | |
3.5.1.110 | ureidoacrylate peracid + H2O | Escherichia coli JW5139 | - |
peroxyaminoacrylate + NH3 + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.46 | Escherichia coli | P75898 | gene rutA | - |
3.5.1.110 | Escherichia coli | P75897 | K-12, gene rutB | - |
3.5.1.110 | Escherichia coli JW5139 | P75897 | K-12, gene rutB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.99.46 | recombinant His-tagged RutA from ASKA strain JW0997 by nickel affinity chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.46 | additional information | (Z)-3-ureidoacrylic peracid is not stable and decomposes nonenzymatically to give (Z)-3-ureidoacrylate | Escherichia coli | ? | - |
? | |
1.14.99.46 | thymine + FMNH2 + O2 | - |
Escherichia coli | (Z)-2-methylureidoacrylate peracid + FMN | - |
? | |
1.14.99.46 | uracil + FMNH2 + O2 | - |
Escherichia coli | (Z)-3-ureidoacrylate peracid + FMN | - |
? | |
1.14.99.46 | uracil + FMNH2 + O2 | RutA directly cleaves the uracil ring between N-3 and C-4 to yield ureidoacrylate | Escherichia coli | (Z)-3-ureidoacrylate peracid + FMN | product analysis by both NMR spectroscopy and mass spectrometry, overview | ? | |
3.5.1.110 | additional information | in vitro RutB cleaves ureidoacrylate hydrolytically to release 2 mol of ammonium, malonic semialdehyde, and carbon dioxide | Escherichia coli | ? | - |
? | |
3.5.1.110 | additional information | in vitro RutB cleaves ureidoacrylate hydrolytically to release 2 mol of ammonium, malonic semialdehyde, and carbon dioxide | Escherichia coli JW5139 | ? | - |
? | |
3.5.1.110 | ureidoacrylate peracid + H2O | - |
Escherichia coli | peroxyaminoacrylate + NH3 + CO2 | - |
? | |
3.5.1.110 | ureidoacrylate peracid + H2O | - |
Escherichia coli JW5139 | peroxyaminoacrylate + NH3 + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.99.46 | RutA | - |
Escherichia coli |
3.5.1.110 | RutB | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.99.46 | 22 | - |
assay at room temperature | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.99.46 | 7 | 8.2 | assay at | Escherichia coli |
3.5.1.110 | 8.2 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.46 | FAD | FAD works much less well than FMN | Escherichia coli | |
1.14.99.46 | FMN | preferred cofactor | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.99.46 | metabolism | RutA has a central role in the Rut pathway, a pyrimidine catabolic pathway, which is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source, pathway overview. RutA works in conjunction with the flavin reductase RutF | Escherichia coli |
1.14.99.46 | physiological function | RutA has a central role in the Rut pathway, which is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source | Escherichia coli |
3.5.1.110 | metabolism | functions of enzymes encoded in the rut gene cluster, overview | Escherichia coli |