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Literature summary extracted from

  • Xu, Y.; Mortimer, M.W.; Fisher, T.S.; Kahn, M.L.; Brockman, F.J.; Xun, L.
    Cloning, sequencing, and analysis of a gene cluster from Chelatobacter heintzii ATCC 29600 encoding nitrilotriacetate monooxygenase and NADH:flavin mononucleotide oxidoreductase (1997), J. Bacteriol., 179, 1112-1116.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.10 expressed in Escherichia coli JM105 cells Aminobacter aminovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.10 Mg2+ required Aminobacter aminovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.10 34500
-
 x * 34500, calculated from amino acid sequence Aminobacter aminovorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.10 Aminobacter aminovorans P54989 component A of nitrilotriacetate monooxygenase
-
1.14.14.10 Aminobacter aminovorans ATCC 29600 P54989 component A of nitrilotriacetate monooxygenase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.10 additional information the NTA monooxygenase activity requires two component proteins, component A and component B. Component A is an NTA monooxygenase that uses FMNH2 and O2 to oxidize nitrilotriacetate, and component B is an NADH:FMN oxidoreductase that provides FMNH2 for nitrilotriacetate oxidation Aminobacter aminovorans ?
-
 ?
1.14.14.10 additional information the NTA monooxygenase activity requires two component proteins, component A and component B. Component A is an NTA monooxygenase that uses FMNH2 and O2 to oxidize nitrilotriacetate, and component B is an NADH:FMN oxidoreductase that provides FMNH2 for nitrilotriacetate oxidation Aminobacter aminovorans ATCC 29600 ?
-
 ?
1.14.14.10 nitrilotriacetate + FMNH2 + H+ + O2
-
 Aminobacter aminovorans iminodiacetate + glyoxylate + FMN + H2O
-
 ?
1.14.14.10 nitrilotriacetate + FMNH2 + H+ + O2
-
 Aminobacter aminovorans ATCC 29600 iminodiacetate + glyoxylate + FMN + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.10 ? x * 34500, calculated from amino acid sequence Aminobacter aminovorans

Synonyms

EC Number Synonyms Comment Organism
1.14.14.10 NTA monooxygenase
-
 Aminobacter aminovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.10 FMNH2
-
 Aminobacter aminovorans