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Literature summary extracted from

  • Paul, L.; Krzycki, J.
    Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid protein homologous to methionine synthase (1996), J. Bacteriol., 178, 6599-6607.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.251 gene mtsA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha. The gene encoding the alpha subunit of the CoM methylase, mtsA, is 5' to the gene encoding the beta subunit, mtsB, regulated cotranscription of mtsA and mtsB Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.251 480000
-
-
 Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.251 a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + coenzyme M Methanosarcina barkeri
-
 methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein]
-
 ?
2.1.1.251 methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide
-
 ?
2.1.1.251 methanethiol + coenzyme M Methanosarcina barkeri
-
 methyl-CoM + hydrogen sulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.251 Methanosarcina barkeri Q48924 gene mtsA
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.251 a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein] mechanism of corrinoid binding by proteins involved in methylotrophic methanogenesis Methanosarcina barkeri
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.251 additional information the mtsA transcript is most abundant in cells grown to the late log phase on acetate but barely detectable in cells grown on methanol or trimethylamine, overview Methanosarcina barkeri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.251 a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein]
-
 ?
2.1.1.251 methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide
-
 ?
2.1.1.251 methanethiol + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + hydrogen sulfide
-
 ?
2.1.1.251 additional information the 480-kDa protein is not methylated by methyltetrahydrosarcinapterin or demethylated by homocysteine. The alpha subunit, not the beta subunit, methylates the thiol of CoM Methanosarcina barkeri ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.251 methyltransferase II
-
 Methanosarcina barkeri
2.1.1.251 MtsA
-
 Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.251 evolution the sequence of MtsA is homologous to the A and M isozymes of methylcobamide:coenzyme M methyltransferases (methyltransferase II), indicating that the alpha polypeptide is a member of the methyltransferase II family of coenzyme M methylases Methanosarcina barkeri