Any feedback?
Literature summary extracted from
- Catabolic pathway for 2-nitroimidazole involves a novel nitrohydrolase that also confers drug resistance (2011), Environ. Microbiol., 13, 1010-1017.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.99.9 | expressed in Escherichia coli | Mycobacterium sp. |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.99.9 | cytoplasm | - |
Mycobacterium sp. | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.9 | NaCl | 1 mM, stimulation | Mycobacterium sp. |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.9 | 2-nitroimidazole + H2O | Mycobacterium sp. | - |
imidazol-2-one + nitrite | - |
? | |
| 3.5.99.9 | 2-nitroimidazole + H2O | Mycobacterium sp. JS330 | - |
imidazol-2-one + nitrite | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.99.9 | Mycobacterium sp. | F4ZCI3 | - |
- |
| 3.5.99.9 | Mycobacterium sp. JS330 | F4ZCI3 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.9 | 0.011 | - |
substrate benznidazole, pH 7.2, 24°C | Mycobacterium sp. |
| 3.5.99.9 | 0.0236 | - |
substrate 2-nitroimidazole, pH 7.2, 24°C | Mycobacterium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.9 | 2-nitroimidazole + H2O | - |
Mycobacterium sp. | imidazol-2-one + nitrite | - |
? | |
| 3.5.99.9 | 2-nitroimidazole + H2O | - |
Mycobacterium sp. JS330 | imidazol-2-one + nitrite | - |
? | |
| 3.5.99.9 | benznidazole + H2O | - |
Mycobacterium sp. | N-benzyl-2-(2-oxo-2,3-dihydro-1H-imidazol-1-yl)acetamide + nitrite | - |
? | |
| 3.5.99.9 | benznidazole + H2O | - |
Mycobacterium sp. JS330 | N-benzyl-2-(2-oxo-2,3-dihydro-1H-imidazol-1-yl)acetamide + nitrite | - |
? | |
| 3.5.99.9 | additional information | no activity with 4-nitroimidazole, metronidazole, 2,4-dinitroimidazole, 3-nitro-1,2,4-triazol-5-one, nitazoxanide, or tizoxanide | Mycobacterium sp. | ? | - |
? | |
| 3.5.99.9 | additional information | no activity with 4-nitroimidazole, metronidazole, 2,4-dinitroimidazole, 3-nitro-1,2,4-triazol-5-one, nitazoxanide, or tizoxanide | Mycobacterium sp. JS330 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.99.9 | 2NI denitrase | - |
Mycobacterium sp. |
| 3.5.99.9 | 2NI nitrohydrolase | - |
Mycobacterium sp. |
| 3.5.99.9 | NnhA | - |
Mycobacterium sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.9 | 24 | - |
- |
Mycobacterium sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.9 | 7.2 | - |
- |
Mycobacterium sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.9 | additional information | no cofactors required, no oxygen consumption | Mycobacterium sp. |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.5.99.9 | metabolism | Initial step in the biodegradation of 2-nitroimidazole by Mycobacterium sp. JS330. This is the first hydrolytic enzyme involved in elimination of the nitro group. The organism grows on 2-nitroimidazole as sole carbon source. | Mycobacterium sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
