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Literature summary extracted from
- Biosynthesis of trimethylamine oxide in Calanus finmarchicus. Properties of a soluble trimethylamine monooxygenase (1980), Comp. Biochem. Physiol. B, 65, 243-249.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.148 | O2 | when the oxygen in the enzyme assay mixture is replaced with 100% nitrogen, there is no formation of N,N,N-trimethylamine N-oxide, showing that the enzyme is oxygen-dependent | Calanus finmarchicus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.13.148 | addition of FAD and dithiothreitol together with NADPH improves the stability of the enzyme | Calanus finmarchicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.148 | Cu2+ | 0.2 mM Cu2+ inhibits the enzyme strongly without any preincubation | Calanus finmarchicus | |
| 1.14.13.148 | additional information | 10 mM EDTA, 1 mM potassium cyanide and 1 mM sodium azide do not inhibit the enzyme. Bubbling the enzyme solution with CO for 2 min at 0°C or including CO (CO:O2 ratio 1:1) during the tests has no effect on enzyme activity | Calanus finmarchicus | |
| 1.14.13.148 | N-ethylmaleimide | preincubation of the enzyme for 15 min at 14°C, pH 7.7, in the presence of 1.0 mM N-ethylmaleimide inhibits the enzymatic activity approximately by 25% | Calanus finmarchicus | |
| 1.14.13.148 | p-chloromercuribenzoate | preincubation of the enzyme for 15 min at 14°C, pH 7.7, in the presence of 0.2 mM p-chloromercuribenzoate inhibits the enzymic activity approximately by 90% | Calanus finmarchicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.148 | 0.0003 | - |
N,N,N-trimethylamine | in potassium diphosphate buffer (pH 7.7), at 14°C | Calanus finmarchicus | |
| 1.14.13.148 | 0.0008 | - |
NADPH | in potassium diphosphate buffer (pH 7.7), at 14°C | Calanus finmarchicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.148 | cytosol | - |
Calanus finmarchicus | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.148 | additional information | no metal ion activators are found | Calanus finmarchicus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.148 | 130000 | - |
gel filtration | Calanus finmarchicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.148 | Calanus finmarchicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.148 | ammonium sulfate precipitation, Sephadex G-200 gel filtration, DEAE-cellulose column chromatography, and calcium phosphate chromatography | Calanus finmarchicus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.148 | 0.00047 | - |
supernatant of homogenate, in potassium diphosphate buffer (pH 7.7), at 14°C | Calanus finmarchicus |
| 1.14.13.148 | 0.085 | - |
after 180fold purification, in potassium diphosphate buffer (pH 7.7), at 14°C | Calanus finmarchicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.148 | N,N,N-trimethylamine + NADPH + H+ + O2 | highest affinity substrate | Calanus finmarchicus | N,N,N-trimethylamine N-oxide + NADP+ + H2O | - |
? | |
| 1.14.13.148 | N,N-dimethylamine + NADPH + H+ + O2 | - |
Calanus finmarchicus | N,N-dimethylamine N-oxide + NADP+ + H2O | - |
? | |
| 1.14.13.148 | N,N-dimethylaniline + NADPH + H+ + O2 | - |
Calanus finmarchicus | N,N-dimethylaniline N-oxide + NADP+ + H2O | - |
? | |
| 1.14.13.148 | N,N-dimethylbutylamine + NADPH + H+ + O2 | - |
Calanus finmarchicus | N,N-dimethylbutylamine N-oxide + NADP+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.148 | TMA monooxygenase | - |
Calanus finmarchicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.148 | 14 | - |
- |
Calanus finmarchicus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.148 | 10 | 20 | - |
Calanus finmarchicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.148 | - |
20 | the enzyme is stable for 30 min at temperatures between 0 and 20°C, when the enzyme is preincubated for 30 min at temperatures above 20°C, a decrease in enzymic activity ensues | Calanus finmarchicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.148 | 7.7 | 7.8 | - |
Calanus finmarchicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.148 | FAD | - |
Calanus finmarchicus | |
| 1.14.13.148 | NADPH | dependent on, in the absence of NADPH the enzyme is rapidly and irreversibly inactivated at 0-5°C. NADP+ also stabilizes the enzyme, but to a lesser extent than NADPH. No oxidation of N,N,N-trimethylamine is observed when NADH is used in the enzyme assay instead of NADPH | Calanus finmarchicus | |
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Release 2023.1 (January 2023)
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